ArticlePLoS pathogens2018
Cotton Leaf Curl Multan virus C4 protein suppresses both transcriptional and post-transcriptional gene silencing by interacting with SAM synthetase.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed, 125 citations in OpenAlex.
- Characterization of the C4 protein encoded by parsley yellow leaf curl virus.Virology journal · 2026Article
- A viral protein diverts the endocytic VPS9a-Rab5 pathway to promote virus infection in plants.Science advances · 2026Article
- Article
- Reversible S-palmitoylation of C4 protein encoded by TYLCCxV orchestrates geminiviral pathogenesis.Stress biology · 2026Article
- High-throughput sequencing-based virome analysis reveals genomic diversity and recombination of tomato-infecting viruses in Korea.Scientific reports · 2026Article
- Genome-wide identification and functional analysis of the SAMS gene family in peanut reveal the role of Ah6Q1KS5 in resistance to bacterial wilt.BMC plant biology · 2026Article
- Epigenetic and Epitranscriptomic Antiviral Responses in Plants for Disease Management.Viruses · 2025Review
- Whitefly transmitted begomovirus infection in plant and host response: a review.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The NbCBP1-NbSAMS1 Module Promotes Ethylene Accumulation to EnhanceInternational journal of molecular sciences · 2025Article
- Reprogrammed Plant Metabolism During Viral Infections: Mechanisms, Pathways and Implications.Molecular plant pathology · 2025Review
- Characterisation of a Betasatellite Associated With Tomato Yellow Leaf Curl Guangdong Virus and Discovery of an Unusual Modulation of Virus Infection Associated With C4 Protein.Molecular plant pathology · 2025Article
- In Silico Investigation of the Interactions Between Cotton Leaf Curl Multan Virus Proteins and the Transcriptional Gene Silencing Factors of Gossypium hirsutum L.Journal of molecular evolution · 2024Article
- Genetic Dissection of ToLCNDV Resistance in Resistant Sources ofInternational journal of molecular sciences · 2024Article
- Dominance of Cotton leaf curl Multan virus-Rajasthan strain associated with third epidemic of cotton leaf curl disease in Pakistan.Scientific reports · 2024Article
- Dietary vitamin B6 intake and stroke are negatively associated in adults: A cross-sectional study from the NHANES.Heliyon · 2024Article
- The P2 nucleic acid binding protein ofPeerJ · 2024Article
- Transcriptional and epigenetic changes during tomato yellow leaf curl virus infection in tomato.BMC plant biology · 2023Article
- Broad functional landscape of geminivirus-encoded C4/AC4 protein.Crop health · 2023Review
- Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression inPlants (Basel, Switzerland) · 2023Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene silencing is a natural antiviral defense mechanism in plants. For effective infection, plant viruses encode viral silencing suppressors to counter this plant antiviral response. The geminivirus-encoded C4 protein has been identified as a gene silencing suppressor, but the underlying mechanism of action has not been characterized. Here, we report that Cotton Leaf Curl Multan virus (CLCuMuV) C4 protein interacts with S-adenosyl methionine synthetase (SAMS), a core enzyme in the methyl cycle, and inhibits SAMS enzymatic activity. By contrast, an R13A mutation in C4 abolished its capacity to interact with SAMS and to suppress SAMS enzymatic activity. Overexpression of wild-type C4, but not mutant C4R13A, suppresses both transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS). Plants infected with CLCuMuV carrying C4R13A show decreased levels of symptoms and viral DNA accumulation associated with enhanced viral DNA methylation. Furthermore, silencing of NbSAMS2 reduces both TGS and PTGS, but enhanced plant susceptibility to two geminiviruses CLCuMuV and Tomato yellow leaf curl China virus. These data suggest that CLCuMuV C4 suppresses both TGS and PTGS by inhibiting SAMS activity to enhance CLCuMuV infection in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.