ArticlePloS one2018
Inter-kingdom effect on epithelial cells of the N-Acyl homoserine lactone 3-oxo-C12:2, a major quorum-sensing molecule from gut microbiota.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 59 citations in OpenAlex.
- Insights into the activity, production, and signaling circuits of homoserine lactones in mammalian cells.iScience · 2026Article
- Quorum-Sensing Molecules Are Elevated in Long-Standing Ulcerative Colitis and Are Linked to the Development of Colitis-Associated Cancer.Gastroenterology · 2026Article
- The Quorum-Sensing Regulator SdiA ActivatesMicroorganisms · 2026Article
- Narrative review on bacteria-derived metabolites in the pathogenesis of ulcerative colitis.Clinical microbiology reviews · 2025Review
- Leveraging chemical synthesis to discover metabolites from the gut microbiome.Annals of the New York Academy of Sciences · 2025Review
- Quorum Sensing: Not Just a Bridge Between Bacteria.MicrobiologyOpen · 2025Review
- Role of the LuxR solo, SdiA, in eavesdropping on foreign bacteria.FEMS microbiology reviews · 2025Review
- Targeting quorum sensing for manipulation of commensal microbiota.BMC biotechnology · 2024Review
- Understanding Quorum-Sensing and Biofilm Forming in Anaerobic Bacterial Communities.International journal of molecular sciences · 2024Review
- Ultra-processed foods and food additives in gut health and disease.Nature reviews. Gastroenterology & hepatology · 2024Review
- Quorum sensing-related activities of beneficial and pathogenic bacteria have important implications for plant and human health.FEMS microbiology ecology · 2024Review
- Microbiome metabolite quantification methods enabling insights into human health and disease.Methods (San Diego, Calif.) · 2024Review
- Targeting microbial quorum sensing: the next frontier to hinder bacterial driven gastrointestinal infections.Gut microbes · 2023Review
- Quorum sensing modulates bacterial virulence and colonization dynamics of the gastrointestinal pathogenGut microbes · 2023Article
- AmBio · 2023Article
- Anti-Quorum Sensing Activity of Probiotics: The Mechanism and Role in Food and Gut Health.Microorganisms · 2023Review
- Conversations in the Gut: The Role of Quorum Sensing in Normobiosis.International journal of molecular sciences · 2023Review
- Regulation of Physiological Barrier Function by the Commensal Microbiota.Life (Basel, Switzerland) · 2023Review
- Impact of IBD-Associated Dysbiosis on Bacterial Quorum Sensing Mediated by Acyl-Homoserine Lactone in Human Gut Microbiota.International journal of molecular sciences · 2022Article
- Furanone and phytol influence metabolic phenotypes regulated by acyl-homoserine lactone in Salmonella.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
Corrections and comments
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Authors and funding
17 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsN-acyl homoserine lactones (AHLs), which are autoinducer quorum-sensing molecules involved in the bacterial communication network, also interact with eukaryotic cells. Searching for these molecules in the context of inflammatory bowel disease (IBD) is appealing. The aims of our study were to look for AHL molecules in faecal samples from healthy subjects (HS) and IBD patients to correlate AHL profiles with the microbiome and investigate the effect of AHLs of interest on epithelial cells.
methodsUsing mass spectrometry, we characterised AHL profiles in faecal samples from HS (n = 26) and IBD patients in remission (n = 24) and in flare (n = 25) and correlated the presence of AHLs of interest with gut microbiota composition obtained by real-time qPCR and 16S sequencing. We synthesised AHLs of interest to test the inflammatory response after IL1β stimulation and paracellular permeability on Caco-2 cells.
resultsWe observed 14 different AHLs, among which one was prominent. This AHL corresponded to 3-oxo-C12:2 and was found significantly less frequently in IBD patients in flare (16%) and in remission (37.5%) versus HS (65.4%) (p = 0.001). The presence of 3-oxo-C12:2 was associated with significantly higher counts of Firmicutes, especially Faecalbacterium prausnitzii, and lower counts of Escherichia coli. In vitro, 3-oxo-C12:2 exerted an anti-inflammatory effect on Caco-2 cells. Interestingly, although 3-oxo-C12, the well-known AHL from Pseudomonas aeruginosa, increased paracellular permeability, 3-oxo-C12:2 did not.
conclusionsWe identified AHLs in the human gut microbiota and discovered a new and prominent AHL, 3-oxo-C12:2, which correlates with normobiosis and exerts a protective effect on gut epithelial cells.
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