ArticleMolecular biology of the cell2018
On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility.
Article in Molecular biology of the cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed.
- Profilin promotes lamellipodium protrusion by tuning the antagonistic activities of capping protein and VASP.Nature communications · 2026Article
- Research on the mechanism of CRMP4-mediated cytoskeletal actin polymerization in promoting invasive migration of stromal cells in endometriosis.Journal of translational medicine · 2026Article
- Reconstructing Actin Dynamics of the Leading Edge from Observational Data.bioRxiv : the preprint server for biology · 2026Article
- Nance-Horan-syndrome-like 1b controls mesodermal cell migration by regulating protrusion and actin dynamics during zebrafish gastrulation.Communications biology · 2025Article
- Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake.Journal of materials chemistry. B · 2025Article
- Cell-mechanical parameter estimation from 1D cell trajectories using simulation-based inference.PloS one · 2025Article
- WAVE2 Regulates Actin-Dependent Processes Induced by the B Cell Antigen Receptor and Integrins.Cells · 2023Article
- Tensile Overload Injures Human Alveolar Epithelial Cells through YAP/F-Actin/MAPK Signaling.Biomedicines · 2023Article
- On multistability and constitutive relations of cell motion on fibronectin lanes.Biophysical journal · 2023Article
- Roles of the cytoskeleton in human diseases.Molecular biology reports · 2023Review
- ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning.Science advances · 2023Article
- The actin cytoskeleton: Morphological changes in pre- and fully developed lung cancer.Biophysics reviews · 2022Review
- Lamellipodia-like actin networks in cells lacking WAVE regulatory complex.Journal of cell science · 2022Article
- Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration.Nature cell biology · 2021Article
- Nance-Horan Syndrome-like 1 protein negatively regulates Scar/WAVE-Arp2/3 activity and inhibits lamellipodia stability and cell migration.Nature communications · 2021Article
- On the adhesion-velocity relation and length adaptation of motile cells on stepped fibronectin lanes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity.Science immunology · 2020Article
- The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration.Cells · 2020Article
- Article
- Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation.Journal of cell science · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lamellipodia are flat membrane protrusions formed during mesenchymal motion. Polymerization at the leading edge assembles the actin filament network and generates protrusion force. How this force is supported by the network and how the assembly rate is shared between protrusion and network retrograde flow determines the protrusion rate. We use mathematical modeling to understand experiments changing the F-actin density in lamellipodia of B16-F1 melanoma cells by modulation of Arp2/3 complex activity or knockout of the formins FMNL2 and FMNL3. Cells respond to a reduction of density with a decrease of protrusion velocity, an increase in the ratio of force to filament number, but constant network assembly rate. The relation between protrusion force and tension gradient in the F-actin network and the density dependency of friction, elasticity, and viscosity of the network explain the experimental observations. The formins act as filament nucleators and elongators with differential rates. Modulation of their activity suggests an effect on network assembly rate. Contrary to these expectations, the effect of changes in elongator composition is much weaker than the consequences of the density change. We conclude that the force acting on the leading edge membrane is the force required to drive F-actin network retrograde flow.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.