Evidence map›Paper›PMID 30155828›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

In Vivo Applications of Cell-Penetrating Zinc-Finger Transcription Factors.

Chonghua Ren, Alexa N Adams, Benjamin Pyles, Barbara J Bailus, Henriette O'Geen, David J Segal

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Chonghua RenGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.
Alexa N AdamsGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.
Benjamin PylesGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.
Barbara J BailusGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.
Henriette O'GeenGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.
David J SegalGenome Center and Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA. djsegal@ucdavis.edu.
University of California, Davis · USBuck Institute for Research on Aging · US

Funding

UC Davis Clinical and Translational Science CenterUL1TR000002 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BERGLUND, LARS F · 2012 to 2015
$12.2M
Rodent Behavior CoreU54HD079125 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CRAWLEY, JACQUELINE N · 2013 to 2019
$8.5M
Towards Gene Therapy for Angelman SyndromeR21NS071028 · NINDS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SEGAL, DAVID J · 2010 to 2011
$392k
Howard Hughes Medical Institute 56005706NCATS NIH HHS UL1 TR000002NICHD NIH HHS U54 HD079125NINDS NIH HHS R21 NS071028
6 · The paper itself

Abstract

Artificial transcription factors based on zinc finger, TALE, and CRISPR/Cas9 programmable DNA-binding platforms have been widely used to regulate the expression of specific genes in cultured cells, but their delivery into organs such as the brain represents a critical challenge to apply such tools in live animals. In previous work, we developed a zinc-finger-based artificial transcription factor harboring a cell-penetrating peptide (CPP) that could be injected systemically, cross the blood-brain barrier, and alter expression of a specific gene in the brain of an adult mouse. Importantly, our mode of delivery produced widespread distribution throughout the brain. Here we describe methods for the production and purification of the factor, testing CPP activity in cells, and testing CPP activity in mice.

Indexed as

Gene Transfer TechniquesZinc FingersAnimalsBlood-Brain BarrierBrainCell-Penetrating PeptidesFemaleGene Expression RegulationGenetic EngineeringMaleMiceMice, Inbred C57BLTranscription FactorsCell-Penetrating PeptidesTranscription FactorsAnimal modelsArtificial transcription factorsCell-penetrating peptideEngineered zinc-finger proteinTAT peptide

Identifiers

PMID30155828
PMCPMC6296463
OpenAlexW2889065827

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.