ArticleIBRO reports2018
VGAT and VGLUT2 expression in MCH and orexin neurons in double transgenic reporter mice.
Article in IBRO reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Hypothalamic Glutamatergic/Orexinergic Neurons Attenuate Fentanyl-Induced Respiratory Depression via Medullary Pre-BöTzinger Complex Pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Temporal drift of sleep-wake representations in hypothalamic neuronal ensembles.Nature communications · 2026Article
- Glutamatergic Heterogeneity in the Neuropeptide Projections From the Lateral Hypothalamus to the Mouse Olfactory Bulb.The Journal of comparative neurology · 2026Article
- Glutamatergic signaling from melanin-concentrating hormone-producing neurons: A requirement for memory regulation, but not for metabolism control.PNAS nexus · 2024Article
- The Co-Expression Pattern of Calcium-Binding Proteins with γ-Aminobutyric Acid and Glutamate Transporters in the Amygdala of the Guinea Pig: Evidence for Glutamatergic Subpopulations.International journal of molecular sciences · 2023Article
- Neuroplastin exerts antiepileptic effects through binding to the α1 subunit of GABA type A receptors to inhibit the internalization of the receptors.Journal of translational medicine · 2023Article
- Fast neurotransmitter identity of MCH neurons: Do contents depend on context?Frontiers in neuroendocrinology · 2023Review
- α-Synuclein Induced the Occurrence of RBD via Interaction with OX1R and Modulated Its Degradation.Neuromolecular medicine · 2023Article
- Does EGFR Signaling Mediate Orexin System Activity in Sleep Initiation?International journal of molecular sciences · 2023Review
- GRIN lens applications for studying neurobiology of substance use disorder.Addiction neuroscience · 2022Article
- GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig.International journal of molecular sciences · 2022Article
- Multifaceted roles of orexin neurons in mediating methamphetamine-induced changes in body temperature and heart rate.IBRO neuroscience reports · 2022Article
- Identification of miRNA-mRNA regulatory network associated with the glutamatergic system in post-traumatic epilepsy rats.Frontiers in neurology · 2022Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).European journal of immunology · 2021Review
- An overview of the orexinergic system in different animal species.Metabolic brain disease · 2021Review
- Article
- Sleep-Wake Neurobiology.Advances in experimental medicine and biology · 2021Review
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- The role of co-neurotransmitters in sleep and wake regulation.Molecular psychiatry · 2019Review
- A lateral hypothalamus to basal forebrain neurocircuit promotes feeding by suppressing responses to anxiogenic environmental cues.Science advances · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The neuropeptides orexin and melanin-concentrating hormone (MCH), as well as the neurotransmitters GABA (γ-Aminobutyric acid) and glutamate are chief modulators of the sleep-wake states in the posterior hypothalamus. To investigate co-expression of vesicular GABA transporter (VGAT, a marker of GABA neurons) and the vesicular glutamate transporter-2 (VGLUT2, a marker of glutamate neurons) in orexin and MCH neurons, we generated two transgenic mouse lines. One line selectively expressed the reporter gene EYFP in VGAT+ neurons, whereas the other line expressed reporter gene tdTomato in VGLUT2+ neurons. Co-localization between these genetic reporters and orexin or MCH immunofluorescent tags was determined using 3D computer reconstructions of Z stacks that were acquired using a multiphoton laser confocal microscope. Our results demonstrated that MCH neurons expressed neither VGAT nor VGLUT2, suggesting MCH neurons are a separate cluster of cells from VGAT+ GABAergic neurons and VGLUT2+ glutamatergic neurons. Moreover, most orexin neurons expressed VGLUT2, indicating these neurons are glutamatergic. Our data suggested that in the posterior hypothalamus there are four major distinct groups of neurons: VGAT+, orexin+/VGLUT2+, orexin-/VGLUT2+, and MCH neurons. This study facilitated our understanding of the role of these neurotransmitters and neuropeptides in relation to sleep/wake regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.