ArticleChemical science2016
Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- High-Fidelity, One-Pot Nucleic Acid Amplification via OMEGA IsrB Nickase Cycling for Clinical Pathogen Detection.JACS Au · 2025Article
- Adaptation of bacterial natural single guide RNA (tracr-L) for efficient plant genome editing.Plant cell reports · 2024Article
- Modulation of Equid Herpesvirus-1 Replication DynamicsViruses · 2024Article
- A domino-like localized cascade toehold assembly amplification-based DNA nanowire for microRNA imaging in living cells.Chemical science · 2022Article
- CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging.Chemical science · 2022Article
- Review
- Programmable Biosensors Based on RNA-Guided CRISPR/Cas Endonuclease.Biological procedures online · 2022Review
- A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases.Chemical science · 2021Article
- CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules.Chemical science · 2021Review
- Improved nearest-neighbor parameters for the stability of RNA/DNA hybrids under a physiological condition.Nucleic acids research · 2020Article
- Versatile in vitro assay to recognize Cas9-induced mutations.Plant direct · 2020Article
- Article
- Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level.Chemical science · 2020Review
- Colorimetric detection of nucleic acid sequences in plant pathogens based on CRISPR/Cas9 triggered signal amplification.Mikrochimica acta · 2019Article
- A universal method for sensitive and cell-free detection of CRISPR-associated nucleases.Chemical science · 2019Article
- A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection.Nature communications · 2018Article
- Construction of an autonomously concatenated hybridization chain reaction for signal amplification and intracellular imaging.Chemical science · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR/Cas9 is a highly efficient genome engineering tool, yet its off-target effects and sequence-dependent cleavage activity across different sgRNAs remain major concerns for its application. Here, we propose a nicking triggered exponential amplification reaction (NTEXPAR), a fast and sensitive
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.