ArticleChemical science2016
A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection.
Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- A Caspase-3-Activatable Near-Infrared AIEgen for Tumor Apoptosis Imaging In Vivo.Chemical & biomedical imaging · 2026Article
- Advancements in Biosensors Based on the Assembles of Small Organic Molecules and Peptides.Biosensors · 2023Review
- Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.Molecules (Basel, Switzerland) · 2023Article
- Development of Small-Molecule Fluorescent Probes Targeting Enzymes.Molecules (Basel, Switzerland) · 2022Review
- Progress on Multifunction Enzyme-Activated Organic Fluorescent Probes for Bioimaging.Frontiers in chemistry · 2022Review
- Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.Chemical science · 2021Article
- Enzyme-activated probes in optical imaging: a focus on atherosclerosis.Dalton transactions (Cambridge, England : 2003) · 2021Review
- Fluorescent probes for visualizing ROS-associated proteins in disease.Chemical science · 2021Review
- Modulation of Aggregation-Induced Emission by Excitation Energy Transfer: Design and Application.Topics in current chemistry (Cham) · 2021Review
- Coumarin as a structural component of substrates and probes for serine and cysteine proteases.Biochimica et biophysica acta. Proteins and proteomics · 2020Review
- Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents.Chemical Society reviews · 2020Review
- Rational design of red AIEgens with a new core structure from non-emissive heteroaromatics.Chemical science · 2018Article
- Theranostics based on AIEgens.Theranostics · 2018Review
- Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time-Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Visualization of drug delivery processes using AIEgens.Chemical science · 2017Article
- Development of a novel lysosome-targetable time-gated luminescence probe for ratiometric and luminescence lifetime detection of nitric oxideChemical science · 2017Article
- Self-powered fluorescence display devices based on a fast self-charging/recharging battery (Mg/Prussian blue).Chemical science · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The accurate detection of biological substances is highly desirable to study various biological processes and evaluate disease progression. Herein, we report a self-validated fluorescent probe which is composed of a coumarin fluorophore as the energy donor and a fluorogen with aggregation-induced emission characteristics (AIEgen) as the energy quencher linked through a caspase-3 specific peptide substrate. Unlike the traditionally widely studied fluorescence resonance energy transfer (FRET) probes, our new generation of FRET probe is non-fluorescent itself due to the energy transfer as well as the dissipation of the acceptor energy through the free molecular motion of AIEgen. Upon interaction with caspase-3, the probe displays strong green and red fluorescent signals synchronously due to the separation of the donor-quencher and aggregation of the released AIEgen. The fluorescence turn-on with dual signal amplification allows real-time and self-validated enzyme detection with a high signal-to-background ratio, providing a good opportunity to accurately monitor various biological processes in a real-time manner.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.