ArticleScientific reports2018
Acoustic Tweezing Cytometry Induces Rapid Initiation of Human Embryonic Stem Cell Differentiation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Acoustic Bubble Sensing Techniques and Bioapplications.Biosensors · 2026Review
- Nanoscale acoustic oscillator for mechanoimmunology: NAOMI.Science advances · 2025Article
- Acoustic technologies for the orchestration of cellular functions for therapeutic applications.Science advances · 2025Review
- Influence of cell shape on sonoporation efficiency in microbubble-facilitated delivery using micropatterned cell arrays.Scientific reports · 2024Article
- Metabolic-Glycoengineering-Enabled Molecularly Specific Acoustic Tweezing Cytometry for Targeted Mechanical Stimulation of Cell Surface Sialoglycans.Angewandte Chemie (International ed. in English) · 2024Article
- Acoustic vibration promotes in vitro expansion of human embryonic stem cells.American journal of stem cells · 2024Article
- Rapid responses of human pluripotent stem cells to cyclic mechanical strains applied to integrin by acoustic tweezing cytometry.Scientific reports · 2023Article
- Low-intensity pulsed ultrasound/nanomechanical force generators enhance osteogenesis of BMSCs through microfilaments and TRPM7.Journal of nanobiotechnology · 2022Article
- Engineering multiscale structural orders for high-fidelity embryoids and organoids.Cell stem cell · 2022Review
- Haralick's texture analysis to predict cellular proliferation on randomly oriented electrospun nanomaterials.Nanoscale advances · 2022Article
- Unveiling the morphogenetic code: A new path at the intersection of physical energies and chemical signaling.World journal of stem cells · 2021Review
- Acoustic tweezing of microparticles in microchannels with sinusoidal cross sections.Scientific reports · 2021Article
- Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.Reproductive biology and endocrinology : RB&E · 2021Article
- Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation.Scientific reports · 2020Article
- Combining Biologically Active β-Lactams Integrin Agonists with Poly(l-lactic acid) Nanofibers: Enhancement of Human Mesenchymal Stem Cell Adhesion.Biomacromolecules · 2020Article
- Probing Single-Cell Mechanical Allostasis Using Ultrasound Tweezers.Cellular and molecular bioengineering · 2019Article
- Acoustic tweezing cytometry for mechanical phenotyping of macrophages and mechanopharmaceutical cytotripsy.Scientific reports · 2019Article
- Acoustic Actuation of Integrin-Bound Microbubbles for Mechanical Phenotyping during Differentiation and Morphogenesis of Human Embryonic Stem Cells.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Biomechanical Characterization at the Cell Scale: Present and Prospects.Frontiers in physiology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Mechanical forces play critical roles in influencing human embryonic stem cell (hESC) fate. However, it remains largely uncharacterized how local mechanical forces influence hESC behavior in vitro. Here, we used an ultrasound (US) technique, acoustic tweezing cytometry (ATC), to apply targeted cyclic subcellular forces to hESCs via integrin-bound microbubbles (MBs). We found that ATC-mediated cyclic forces applied for 30 min to hESCs near the edge of a colony induced immediate global responses throughout the colony, suggesting the importance of cell-cell connection in the mechanoresponsiveness of hESCs to ATC-applied forces. ATC application generated increased contractile force, enhanced calcium activity, as well as decreased expression of pluripotency transcription factors Oct4 and Nanog, leading to rapid initiation of hESC differentiation and characteristic epithelial-mesenchymal transition (EMT) events that depend on focal adhesion kinase (FAK) activation and cytoskeleton (CSK) tension. These results reveal a unique, rapid mechanoresponsiveness and community behavior of hESCs to integrin-targeted cyclic forces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.