ArticleExperimental & molecular medicine2018
Structured illumination microscopy imaging reveals localization of replication protein A between chromosome lateral elements during mammalian meiosis.
Article in Experimental & molecular medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Biallelic BRF2 mutations disrupt redox homeostasis as etiological factors in syndromic immunodeficiency and developmental disorders.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Recombination plasticity in response to temperature variation in reptiles.PLoS genetics · 2025Article
- Recombination-coupled DNA synthesis facilitates post-invasion steps in meiotic crossover and noncrossover formations.Nucleic acids research · 2025Article
- FIGNL1-FIRRM is essential for meiotic recombination and prevents DNA damage-independent RAD51 and DMC1 loading.Nature communications · 2024Article
- Formation and resolution of meiotic chromosome entanglements and interlocks.Journal of cell science · 2024Review
- RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination.Nucleic acids research · 2024Article
- Computational Tools for the Analysis of Meiotic Prophase I Images.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Meiotic chromosome organization and its role in recombination and cancer.Current topics in developmental biology · 2023Review
- Extending resolution within a single imaging frame.Nature communications · 2022Article
- Super-Resolution Microscopy of the Synaptonemal Complex within the Caenorhabditis elegans Germline.Journal of visualized experiments : JoVE · 2022Article
- Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis.Communications biology · 2022Article
- Oligopaint DNA FISH reveals telomere-based meiotic pairing dynamics in the silkworm, Bombyx mori.PLoS genetics · 2021Article
- High Resolution View on the Regulation of Recombinase Accumulation in Mammalian Meiosis.Frontiers in cell and developmental biology · 2021Review
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
- Therapeutic Dose of Hydroxyurea-Induced Synaptic Abnormalities on the Mouse Spermatocyte.Frontiers in physiology · 2021Article
- Ring 1 and YY1 Binding Protein Expressed in Murine Spermatocytes but Dispensable for Spermatogenesis.Genes · 2020Article
- E2F1 facilitates DNA break repair by localizing to break sites and enhancing the expression of homologous recombination factors.Experimental & molecular medicine · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An important event enabling meiotic prophase I to proceed is the close juxtaposition of conjoined chromosome axes of homologs and their assembly via an array of transverse filaments and meiosis-specific axial elements into the synaptonemal complex (SC). During meiosis, recombination requires the establishment of a platform for recombinational interactions between the chromosome axes and their subsequent stabilization. This is essential for ensuring crossover recombination and proper segregation of homologous chromosomes. Thus, well-established SCs are essential for supporting these processes. The regulation of recombination intermediates on the chromosome axis/SC and dynamic positioning of double-strand breaks are not well understood. Here, using super-resolution microscopy (structured illumination microscopy), we determined the localization of the replication protein A (RPA) complex on the chromosome axes in the early phase of leptonema/zygonema and within the CEs of SC in the pachynema during meiotic prophase in mouse spermatocytes. RPA, which marks the intermediate steps of pairing and recombination, appears in large numbers and is positioned on the chromosome axes at the zygonema. In the pachynema, RPA foci are reduced but do not completely disappear; instead, they are placed between lateral elements. Our results reveal the precise structure of SC and localization dynamics of recombination intermediates on meiocyte chromosomes undergoing homolog pairing and meiotic recombination.
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