ArticleBlood2018
Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death.
Article in Blood, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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Who cites it
98 citing papers in PubMed, 183 citations in OpenAlex.
- Eosinophil biology in COPD: from scientific insight to clinical treatment action.ERJ open research · 2026Review
- Mechanisms of Eosinophil Degranulation.Cells · 2026Review
- A prognostic model for head and neck squamous cell carcinoma based on eosinophil extracellular trap related genes.Translational cancer research · 2026Article
- Severe Eosinophilic Asthma: From Immunopathology to Pharmacological Treatment.Journal of clinical medicine · 2026Review
- Mucus Plugs and Eosinophilic Inflammation: Expanding the Paradigm to COPD.The journal of allergy and clinical immunology. In practice · 2026Review
- Crystalline Insights into Nasal Mucosa Inflammation and Remodeling: Unveiling Role of Galectin-10.Biomolecules · 2026Article
- Plastic bronchitis: a narrative review of the classification methods and pathogenesis.Frontiers in pediatrics · 2026Review
- Eosinophil-associated intestinal immune responses following SARS-CoV-2 infection in K18-hACE2 mice.PloS one · 2026Article
- The emerging roles of eosinophils in immune regulation in health and disease.Frontiers in immunology · 2026Review
- Eosinophil ETosis in severe non-IgE-mediated gastrointestinal food allergy, a neonatal necrotizing enterocolitis: A case report.Asia Pacific allergy · 2025Article
- Review
- Review
- The immunology of asthma.Nature immunology · 2025Review
- Fatal Eosinophilia-Associated Thrombotic Microangiopathy and the Role of Renal Charcot-Leyden Crystals.Kidney international reports · 2025Article
- IgG4-related disease with eosinophil extracellular traps.QJM : monthly journal of the Association of Physicians · 2025Article
- Accumulation of long-chain unsaturated fatty acids in the airway inflammatory microenvironment drives eosinophil etosis and corticosteroid resistance.Cell communication and signaling : CCS · 2025Article
- Eosinophil ETosis and Charcot-Leyden crystals in Kimura disease.The journal of allergy and clinical immunology. Global · 2025Article
- Treatment of allergic bronchopulmonary aspergillosis with biologics.Chinese medical journal pulmonary and critical care medicine · 2025Review
- Allergic Bronchopulmonary Aspergillosis/Mycosis: An Allergic Disease or an Eosinophilic Disease?Internal medicine (Tokyo, Japan) · 2025Review
- Crowding and in-Cell Crystallization.Sub-cellular biochemistry · 2025Review
38 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
Protein crystallization in human tissue rarely occurs. Charcot-Leyden crystals (CLCs) were described in various eosinophilic diseases >150 years ago, but our understanding of CLC formation still remains limited. In this study, we demonstrate that CLCs observed in varied inflamed human tissues are closely associated with eosinophil cell-free granules and nuclear envelope/plasma membrane disintegration with release of filamentous chromatin (extracellular traps), typical morphologies of a regulated pathway of extracellular trap cell death (ETosis). During the process of eosinophil ETosis, eccentrically localized cytoplasmic and perinuclear CLC protein (galectin-10) is homogeneously redistributed in the cytoplasm. Rapid (1-2 minutes) formation of intracytoplasmic CLCs was observed using time-lapse imaging. Plasma membrane rupture enabled the release of both intracellularly formed CLCs and soluble galectin-10 that further contributed to formation of CLCs extracellularly, in parallel with the expulsion of free intact granules and extracellular traps. CLC formation and galectin-10 release were dependent on nicotinamide adenine dinucleotide phosphate oxidase activation. To our knowledge, this is the first demonstration of natural formation of CLCs in association with an active physiological process (ie, ETosis). These results indicate that dynamic changes in intracellular localization and release of galectin-10 contribute to CLC formation in vivo and suggest that CLC/galectin-10 might serve as an indicator of ETosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.