ArticleMolecular microbiology2018
Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Genome Evolution in Plant Pathogenic Bacteria.Genome biology and evolution · 2026Review
- Pathological convergence of a bacterial plant pathogen is associated with the horizontal transfer of an effector-containing mobile element.BMC genomics · 2026Article
- From gene-for-gene to NLRome-for-effectorome: decoding the dynamic interplay between phytopathogenic effector networks and plant immune systems.FEMS microbiology reviews · 2026Review
- The Role of Genomic Islands in the Pathogenicity and Evolution of Plant-Pathogenic Gammaproteobacteria.Microorganisms · 2025Review
- Diverse Genetic Mechanisms Enable Pseudomonas syringae to Rapidly Overcome Effector-Triggered Immunity.Molecular plant pathology · 2025Article
- Rapid dissemination of host metabolism-manipulating genes via integrative and conjugative elements.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bioinformatic and experimental characterization of SEN1998: a conserved gene carried by the Enterobacteriaceae-associated ROD21-like family of genomic islands.Scientific reports · 2022Article
- What makes a megaplasmid?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Genome Context Influences Evolutionary Flexibility of Nearly Identical Type III Effectors in Two Phytopathogenic Pseudomonads.Frontiers in microbiology · 2022Article
- Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection.PLoS pathogens · 2019Article
- DNA Supercoiling: an Ancestral Regulator of Gene Expression in Pathogenic Bacteria?Computational and structural biotechnology journal · 2019Review
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 is excised from the bacterial chromosome, avrPphB is downregulated and therefore the pathogen avoids triggering the host's defence mechanism. Here, we investigate whether the downregulation of avrPphB is caused by the supercoiling of PPHGI-1. We also investigate the effect of a PPHGI-1-encoded type 1A topoisomerase, TopB3, on island stability and bacterial pathogenicity in the plant. Supercoiling inhibitors significantly increased the expression of avrPphB but did not affect the excision of PPHGI-1. An insertional mutant of topB3 displayed an increase in avrPphB expression and an increase in PPHGI-1 excision as well as reduced population growth in resistant and susceptible cultivars of bean. These results suggest an important role for topoisomerases in the maintenance and stability of a bacterial-encoded genomic island and demonstrate that supercoiling is involved in the downregulation of an effector gene once the island has been excised, allowing the pathogen to prevent further activation of the host defence response.
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