ArticleEvolution; international journal of organic evolution2018
Gain-of-function experiments with bacteriophage lambda uncover residues under diversifying selection in nature.
Article in Evolution; international journal of organic evolution, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 42 citations in OpenAlex.
- Factors Affecting Phage-Bacteria Coevolution Dynamics.Viruses · 2025Review
- Inferring strain-level mutational drivers of phage-bacteria interaction phenotypes arising during coevolutionary dynamics.bioRxiv : the preprint server for biology · 2024Article
- More evolvable bacteriophages better suppress their host.Evolutionary applications · 2024Article
- Structural mechanism of bacteriophage lambda tail's interaction with the bacterial receptor.Nature communications · 2024Article
- Competition-driven eco-evolutionary feedback reshapes bacteriophage lambda's fitness landscape and enables speciation.Nature communications · 2024Article
- Bacteriophage Φ21's receptor-binding protein evolves new functions through destabilizing mutations that generate non-genetic phenotypic heterogeneity.Virus evolution · 2024Article
- Inferring strain-level mutational drivers of phage-bacteria interaction phenotypes arising during coevolutionary dynamics.Virus evolution · 2024Article
- Host-parasite coevolution promotes innovation through deformations in fitness landscapes.eLife · 2022Article
- Leapfrog dynamics in phage-bacteria coevolution revealed by joint analysis of cross-infection phenotypes and whole genome sequencing.Ecology letters · 2022Article
- Viral protein instability enhances host-range evolvability.PLoS genetics · 2022Article
- Balance between promiscuity and specificity in phage λ host range.The ISME journal · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Viral gain-of-function mutations frequently evolve during laboratory experiments. Whether the specific mutations that evolve in the lab also evolve in nature and whether they have the same impact on evolution in the real world is unknown. We studied a model virus, bacteriophage λ, that repeatedly evolves to exploit a new host receptor under typical laboratory conditions. Here, we demonstrate that two residues of λ's J protein are required for the new function. In natural λ variants, these amino acid sites are highly diverse and evolve at high rates. Insertions and deletions at these locations are associated with phylogenetic patterns indicative of ecological diversification. Our results show that viral evolution in the laboratory mirrors that in nature and that laboratory experiments can be coupled with protein sequence analyses to identify the causes of viral evolution in the real world. Furthermore, our results provide evidence for widespread host-shift evolution in lambdoid viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.