ArticlePlant cell reports2018
OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice.
Article in Plant cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Regulatory factors of starch metabolism in cereal endosperm.Plant molecular biology · 2026Review
- TKC-MC: An Effective Strategy for Generating Heritable Heterozygous Mutations in Essential Genes in Rice.Plant biotechnology journal · 2026Article
- Extensin-like Protein OsPEX1 Modulates Grain Filling in Rice.Plants (Basel, Switzerland) · 2025Article
- Natural variation of WBR7 confers rice high yield and quality by modulating sucrose supply in sink organs.Plant biotechnology journal · 2024Article
- Phenotypic Analysis and Gene Cloning of Rice Floury Endosperm MutantPlants (Basel, Switzerland) · 2024Article
- FgCWM1 modulates TaNDUFA9 to inhibit SA synthesis and reduce FHB resistance in wheat.BMC biology · 2024Article
- Rice Seed Protrusion Quantitative Trait Loci Mapping through Genome-Wide Association Study.Plants (Basel, Switzerland) · 2024Article
- Genome-wide association mapping of quantitative trait loci for chalkiness-related traits in rice (Frontiers in genetics · 2024Article
- Regulation of Grain Chalkiness and Starch Metabolism by FLO2 Interaction Factor 3, a bHLH Transcription Factor inInternational journal of molecular sciences · 2023Article
- Genetic Diversity and Breeding Signatures for Regional Indica Rice Improvement in Guangdong of Southern China.Rice (New York, N.Y.) · 2023Article
- Molecular Cytological Analysis and Specific Marker Development in Wheat-International journal of molecular sciences · 2023Article
- Breeding effects on durum wheat traits detected using GWAS and haplotype block analysis.Frontiers in plant science · 2023Article
- Combined Effects of Different Alleles ofPlants (Basel, Switzerland) · 2022Article
- The CBM48 domain-containing protein FLO6 regulates starch synthesis by interacting with SSIVb and GBSS in rice.Plant molecular biology · 2022Article
- FLOURY ENDOSPERM19 encoding a class I glutamine amidotransferase affects grain quality in rice.Molecular breeding : new strategies in plant improvement · 2021Article
- Genetic and Molecular Factors Determining Grain Weight in Rice.Frontiers in plant science · 2021Review
- Identification and Pleiotropic Effect Analysis ofFrontiers in plant science · 2021Article
- Genetic Control and High Temperature Effects on Starch Biosynthesis and Grain Quality in Rice.Frontiers in plant science · 2021Review
- Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana.Scientific reports · 2020Article
- An embryo lethal transgenic line manifests global expression changes and elevated protein/oil ratios in heterozygous soybean plants.PloS one · 2020Article
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15 authors at 1 institution in 1 country.
Funding
Abstract
key messageLoss of function of a mitochondrial complex I subunit (OsNDUFA9) causes abnormal embryo development and affects starch synthesis by altering the expression of starch synthesis-related genes and proteins. Proton-pumping NADH: ubiquinone oxidoreductase (also called complex I) is thought to be the largest and most complicated enzyme of the mitochondrial respiratory chain. Mutations of complex I subunits have been revealed to link with a number of growth inhibitions in plants. However, the function of complex I subunits in rice remains unclear. Here, we isolated a rice floury endosperm mutant (named flo13) that was embryonic lethal and failed to germinate. Semi-thin sectioning analysis showed that compound starch grain development in the mutant was greatly impaired, leading to significantly compromised starch biosynthesis and decreased 1000-grain weight relative to the wild type. Map-based cloning revealed that FLO13 encodes an accessory subunit of complex I protein (designated as OsNDUFA9). A single nucleotide substitution (G18A) occurred in the first exon of OsNDUFA9, introducing a premature stop codon in the flo13 mutant gene. OsNDUFA9 was ubiquitously expressed in various tissues and the OsNDUFA9 protein was localized to the mitochondria. Quantitative RT-PCR and protein blotting indicated loss of function of OsNDUFA9 altered gene expression and protein accumulation associated with respiratory electron chain complex in the mitochondria. Moreover, transmission electron microscopic analysis showed that the mutant lacked obvious mitochondrial cristae structure in the mitochondria of endosperm cell. Our results demonstrate that the OsNDUFA9 subunit of complex I is essential for embryo development and starch synthesis in rice endosperm.
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