ArticleJournal of lipids2018
A Fast and Simple Qualitative Method for Screening Oleaginous Yeasts on Agar.
Article in Journal of lipids, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Newly IsolatedFoods (Basel, Switzerland) · 2026Article
- Effect of single and combined nitrogen sources on lipid production by Rhodotorula kratochvilovae and Rhodotorula paludigena.AMB Express · 2026Article
- Characterization of a Collection of Natural Oleogenic Yeasts to Identify Promising Producers of Food Oil Analogues.International journal of molecular sciences · 2026Article
- Between FAMEs-Qualitative and Biomass-Quantitative: A review study on oleaginous fungi.Biotechnology notes (Amsterdam, Netherlands) · 2026Review
- Screening Method for the Selection of Oleaginous Yeast-Producing Gold Nanoparticles.International journal of molecular sciences · 2025Article
- Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste.Microbial cell factories · 2025Article
- Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies.Archives of microbiology · 2024Review
- Nitrogen limitation-induced adaptive response and lipogenesis in the Antarctic yeastFrontiers in microbiology · 2024Article
- The history, state of the art and future prospects for oleaginous yeast research.Microbial cell factories · 2021Review
- Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification.Frontiers in microbiology · 2021Article
- Single Cell Oil Production by Oleaginous Yeasts Grown in Synthetic and Waste-Derived Volatile Fatty Acids.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Finding new oleaginous yeasts is of great interest due to their many important applications. Currently available screening procedures are time-consuming, and most of these require liquid cultures. In this work, a new, fast, economical, and simple qualitative method for screening oleaginous yeasts was developed. The fluorescent dye, Rhodamine B, was selected because its fluorescence is directly correlated to lipid content, and no additional steps or special equipment are needed. This method only requires growing the yeasts on dyed agar plates. Under visible light, it is easy to observe that nonpigmented oleaginous yeasts become colored, whereas non-oleaginous yeasts remain uncolored. The developed method is also useful for improving medium composition in specific applications. Moreover, it was also adapted to use alternative carbon sources, such as lignocellulosic materials and glycerol. The developed method was applied to screen 124 recently isolated nonpigmented yeasts on three different carbon sources, namely, glucose, glycerol, and agave bagasse hydrolysate. Five strains were selected as good lipid producers on all tested carbon sources and accumulated over 48% lipids. Furthermore, the assay was adapted to screen reddish-pigmented yeasts. Considering all the above, the developed method has a wide range of applications in the field of microbial oils.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.