ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2018
Selection of reference genes for reverse transcription-qPCR analysis in the biomonitor macrophyte
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The RT-qPCR has been the method used to analyze gene expression in plants but its benefits have not been completely exploited in the field of plants ecotoxicology when used as molecular biomarkers. The correct use of RT-qPCR demands to establish a certain number of reference genes (RG) which are expected to be invariable in their expression although it does not always happen. The main goals of this work were to: (1) analyze the stability of six potential RG, (2) establish the optimum number of RG, (3) select the most suitable RG to be applied in
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