ArticleScientific reports2018
Detection and Categorization of Diarrheagenic Escherichia coli with Auto-microfluidic Thin-film Chip Method.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- High Occurrence of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- What Is behind the Correlation Analysis of DiarrheagenicBiology · 2022Article
- Comparative Analysis of Human and Animal E. coli: Serotyping, Antimicrobial Resistance, and Virulence Gene ProfilingAntibiotics (Basel, Switzerland) · 2022Article
- Innovative next-generation therapies in combating multi-drug-resistant and multi-virulent Escherichia coli isolates: insights from in vitro, in vivo, and molecular docking studies.Applied microbiology and biotechnology · 2022Article
- Culture independent DNA extraction method for bacterial cells concentrated from water.MethodsX · 2022Article
- Multidrug resistance to commonly prescribed antibiotics inThe Pan African medical journal · 2021Article
- Molecular serotyping of diarrheagenicGut microbesArticle
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
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Abstract
Diarrheagenic Escherichia coli (DEC) causes human diarrhea symptom in both healthy and immunocompromised individuals. An auto-microfluidic thin-film chip (AMTC) instrument integrating one-step multiplex PCR (mPCR) with reverse dot blot hybridization (RDBH) was developed for high-throughput detection of DEC. The novel mPCR method was developed by designing 14 specific primers and corresponding probes. 14 indexes including an endogenous gene (uidA) and 13 pathogenic genes (stx1, stx2, escV, ipaH, invE, estB, lt, pic, aggR, astA, bfpB, sth and stp) of DEC were detected. This one-step mPCR + RDBH approach is useful for simultaneous detection of numerous target genes in a single sample, whose specificity and availability have been confirmed on the positive control of 11 DEC strains. In addition, with 300 diarrheal stool samples being detected by this method, 21 were found to contain five major DEC strains. Compared with monoplex PCR and previous one-step mPCR approach, this method could detect ipaH and estB, and compared with current commercial kit, the relevance ratio of DEC detected by the AMTC method was increased by 1% in stool samples. Furthermore, the novel integration AMTC device could be a valuable detection tool for categorization of E. coli.
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