ArticleMethods in enzymology2018
Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases.
Article in Methods in enzymology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Chemical Reaction Models in Synthetic Promoter Design in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Exploring the mono-/bistability range of positively autoregulated signaling systems in the presence of competing transcription factor binding sites.PLoS computational biology · 2022Article
- Membrane Sensor Histidine Kinases: Insights from Structural, Ligand and Inhibitor Studies of Full-Length Proteins and Signalling Domains for Antibiotic Discovery.Molecules (Basel, Switzerland) · 2021Review
- Control of theEcoSal Plus · 2019Review
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Quantitation of two-component protein activities is becoming increasingly important to understand the general design principles for this widely distributed prokaryotic signaling pathway. In many two-component systems (TCSs), phosphatase activity of the sensor histidine kinase (HK) plays a major role in controlling the system output and resetting the system to the prestimulus state. Quantitation of the phosphatase activity is often carried out in vitro, usually with truncated proteins that may not recapitulate the intact HK in the cellular environment. This chapter outlines a method for characterizing the intracellular phosphatase activity by investigating the TCS deactivation dynamics upon stimulus withdrawal. Two experimental approaches, the direct Phos-tag gel analysis and the indirect reporter assay, are described for measuring the TCS deactivation dynamics in cell. Combined with a mathematic model, the experimentally determined kinetics can lead to proper evaluation of the intracellular phosphatase activity.
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Registered trials
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