Evidence map›Paper›PMID 30149490›Full record

ArticleJournal of X-ray science and technology2018

Discrimination of DNA and RNA distribution in a mammalian cell by scanning transmission soft X-ray microscopy.

Kunio Shinohara, Atsushi Ito, Takuji Ohigashi, Masataka Kado, Shigenobu Toné

Abstract read
In one paragraph

Article in Journal of X-ray science and technology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Kunio ShinoharaSchool of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan.
Atsushi ItoSchool of Engineering, Tokai University, Hiratsuka, Kanagawa, Japan.
Takuji OhigashiUVSOR Synchrotron, Institute for Molecular Science, Okazaki, Aichi, Japan.
Masataka KadoKansai Photon Science Institute, QST, Kizugawa, Kyoto, Japan.
Shigenobu TonéSchool of Science and Engineering, Tokyo Denki University, Hatoyama, Saitama, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Soft X-ray spectromicroscopy was applied to study the distribution of DNA and RNA in a mammalian cell at the spatial resolution of 400 nm. The relative distribution of DNA and RNA was examined by the SVD (singular value decomposition) method in aXis2000 program using combined full spectra of DNA and RNA at the absorption edge regions of carbon, nitrogen and oxygen. The absorption of nucleic acid was evaluated using 1s-π* transitions in the NEXAFS spectra at the nitrogen K absorption edge and distributed to DNA and RNA according to the relative level obtained above. The present results revealed the usefulness of the SVD method to discriminate closely related molecules such as DNA and RNA.

Indexed as

AlgorithmsAnimalsCHO CellsCricetulusCytological TechniquesDNAMicroscopyMolecular ImagingRNAX-Ray Absorption SpectroscopyDNARNAcultured mammalian cellDNA and RNA mappingquantitative studySTXM

Identifiers

PMID30149490
PMCPMC6311369

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.