ArticlePLoS genetics2018
Numerous recursive sites contribute to accuracy of splicing in long introns in flies.
Article in PLoS genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Pervasive noise in human pre-mRNA splice site selection.Molecular cell · 2026Article
- Detection of a sequence feature for recursive splicing.bioRxiv : the preprint server for biology · 2026Article
- Recursive splicing-a mechanism of intron removal with an unexplored role in the largest genomes.Journal of molecular evolution · 2025Review
- Accurate quantification of nascent and mature RNAs from single-cell and single-nucleus RNA-seq.Nucleic acids research · 2025Article
- The unusual gene architecture of polyubiquitin is created by dual-specific splice sites.Genome biology · 2024Article
- Global impact of aberrant splicing on human gene expression levels.bioRxiv : the preprint server for biology · 2023Article
- Recursive splicing discovery using lariats in total RNA sequencing.Life science alliance · 2023Article
- Exon definitive regions forMolecular therapy. Nucleic acids · 2023Article
- Transcription and splicing dynamics during earlyRNA (New York, N.Y.) · 2022Article
- Molecular and genetic dissection of recursive splicing.Life science alliance · 2022Article
- Recursive splicing is a rare event in the mouse brain.PloS one · 2022Article
- Differential fates of introns in gene expression due to global alternative splicing.Human genetics · 2022Review
- Co-transcriptional splicing regulates 3' end cleavage during mammalian erythropoiesis.Molecular cell · 2021Article
- A novel role of U1 snRNP: Splice site selection from a distance.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
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Authors and funding
5 authors.
Funding
Abstract
Recursive splicing, a process by which a single intron is removed from pre-mRNA transcripts in multiple distinct segments, has been observed in a small subset of Drosophila melanogaster introns. However, detection of recursive splicing requires observation of splicing intermediates that are inherently unstable, making it difficult to study. Here we developed new computational approaches to identify recursively spliced introns and applied them, in combination with existing methods, to nascent RNA sequencing data from Drosophila S2 cells. These approaches identified hundreds of novel sites of recursive splicing, expanding the catalog of recursively spliced fly introns by 4-fold. A subset of recursive sites were validated by RT-PCR and sequencing. Recursive sites occur in most very long (> 40 kb) fly introns, including many genes involved in morphogenesis and development, and tend to occur near the midpoints of introns. Suggesting a possible function for recursive splicing, we observe that fly introns with recursive sites are spliced more accurately than comparably sized non-recursive introns.
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