Evidence map›Paper›PMID 30148852›Full record

ArticlePloS one2018

Errors in protein synthesis increase the level of saturated fatty acids and affect the overall lipid profiles of yeast.

Ana Rita D Araújo, Tânia Melo, Elisabete A Maciel, Clara Pereira, Catarina M Morais, Deolinda R Santinha, Joana F Tavares, Helena Oliveira, Amália S Jurado, Vítor Costa and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Comprehensive Response ofMicroorganisms · 2023
    Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ana Rita D AraújoDepartment of Medical Sciences and Institute of Biomedicine-iBiMED, University of Aveiro, Aveiro, Portugal.
Tânia MeloMass Spectrometry Center, Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal.
Elisabete A MacielMass Spectrometry Center, Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal.
Clara Pereirai3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Catarina M MoraisCNC, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Deolinda R SantinhaMass Spectrometry Center, Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal.
Joana F TavaresDepartment of Medical Sciences and Institute of Biomedicine-iBiMED, University of Aveiro, Aveiro, Portugal.ORCID 0000-0001-7083-6266
Helena OliveiraLaboratory of Biotechnology and Cytomics, Department of Biology, CESAM, University of Aveiro, Aveiro, Portugal.
Amália S JuradoCNC, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Vítor Costai3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Pedro DominguesMass Spectrometry Center, Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal.
Maria Rosário M DominguesMass Spectrometry Center, Department of Chemistry, QOPNA, University of Aveiro, Aveiro, Portugal.
Manuel A S SantosDepartment of Medical Sciences and Institute of Biomedicine-iBiMED, University of Aveiro, Aveiro, Portugal.ORCID 0000-0002-0437-1146

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The occurrence of protein synthesis errors (mistranslation) above the typical mean mistranslation level of 10-4 is mostly deleterious to yeast, zebrafish and mammal cells. Previous yeast studies have shown that mistranslation affects fitness and deregulates genes related to lipid metabolism, but there is no experimental proof that such errors alter yeast lipid profiles. We engineered yeast strains to misincorporate serine at alanine and glycine sites on a global scale and evaluated the putative effects on the lipidome. Lipids from whole cells were extracted and analysed by thin layer chromatography (TLC), liquid chromatography-mass spectrometry(LC-MS) and gas chromatography (GC). Oxidative damage, fatty acid desaturation and membrane fluidity changes were screened to identify putative alterations in lipid profiles in both logarithmic (fermentative) and post-diauxic shift (respiratory) phases. There were alterations in several lipid classes, namely lyso-phosphatidylcholine, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and triglyceride, and in the fatty acid profiles, namely C16:1, C16:0, C18:1 and C18:0. Overall, the relative content of lipid species with saturated FA increased in detriment of those with unsaturated fatty acids. The expression of the OLE1 mRNA was deregulated, but phospholipid fluidity changes were not observed. These data expand current knowledge of mistranslation biology and highlight its putative roles in human diseases.

Indexed as

Protein BiosynthesisFatty AcidsSaccharomyces cerevisiaeStearoyl-CoA Desaturasedelta-9 fatty acid desaturaseFatty AcidsStearoyl-CoA Desaturase

Identifiers

PMID30148852
PMCPMC6110467

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.