ArticleBioconjugate chemistry2018
Chemical Labeling and Affinity Capture of Inosine-Containing RNAs Using Acrylamidofluorescein.
Article in Bioconjugate chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- ENDONUCLEASE V: FROM TRANSCRIPTOME REGULATOR TO CHEMICAL BIOLOGY TOOL.ChemistryEurope · 2026Article
- Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome.Nature methods · 2024Article
- Spatial Visualization of A-to-I Editing in Cells Using Endonuclease V Immunostaining Assay (EndoVIA).ACS central science · 2024Article
- Identification and Interpretation of A-to-I RNA Editing Events in Insect Transcriptomes.International journal of molecular sciences · 2023Review
- Transcriptome-wide profiling of A-to-I RNA editing by Slic-seq.Nucleic acids research · 2023Article
- Forced Intercalation Peptide Nucleic Acid Probes for the Detection of an Adenosine-to-Inosine Modification.ACS omega · 2023Article
- Harnessing Nature's Molecular Recognition Capabilities to Map and Study RNA Modifications.Accounts of chemical research · 2022Article
- Protein-based molecular recognition tools for detecting and profiling RNA modifications.Current opinion in structural biology · 2021Review
- Analysis of RNA Modifications by Second- and Third-Generation Deep Sequencing: 2020 Update.Genes · 2021Review
- Chemical Profiling of A-to-I RNA Editing Using a Click-Compatible Phenylacrylamide.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- EndoVIPER-seq for Improved Detection of A-to-I Editing Sites in Cellular RNA.Current protocols in chemical biology · 2020Article
- Epitranscriptomic marks: Emerging modulators of RNA virus gene expression.Wiley interdisciplinary reviews. RNA · 2020Review
- Selective Enrichment of A-to-I Edited Transcripts from Cellular RNA Using Endonuclease V.Journal of the American Chemical Society · 2020Article
- A-to-I RNA Editing in Cancer: From Evaluating the Editing Level to Exploring the Editing Effects.Frontiers in oncology · 2020Review
- Identification of Adenosine-to-Inosine RNA Editing with Acrylonitrile Reagents.Organic letters · 2019Article
- Experimental and Computational Tools for Mapping Circular RNA Modifications.Wiley interdisciplinary reviews. RNAReview
- Advances in Detection Methods for A-to-I RNA Editing.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adenosine-to-inosine (A-to-I) RNA editing is a widespread and conserved post-transcriptional modification, producing significant changes in cellular function and behavior. Accurately identifying, detecting, and quantifying these sites in the transcriptome is necessary to improve our understanding of editing dynamics, its broader biological roles, and connections with diseases. Chemical labeling of edited bases coupled with affinity enrichment has enabled improved characterization of several forms of RNA editing. However, there are no approaches currently available for pull-down of inosines. To address this need, we explore acrylamide as a labeling motif and report here an acrylamidofluorescein reagent that reacts with inosine and enables enrichment of inosine-containing RNA transcripts. This method provides improved sensitivity in the detection and identification of inosines toward a more comprehensive transcriptome-wide analysis of A-to-I editing. Acrylamide derivatization is also highly generalizable, providing potential for the labeling of inosine with a wide variety of probes and affinity handles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.