Evidence map›Paper›PMID 30147273›Full record

ArticleVeterinary world2018

Use of molecular biology tools for rapid identification and characterization of

Ashraf M Abbas, Dalia A M Abd El-Moaty, Eman S A Zaki, Elham F El-Sergany, Nadine A El-Sebay, Hala A Fadl, A A Samy

Open access · goldAbstract read
In one paragraph

Article in Veterinary world, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Molecular Detection and Characterization ofInternational journal of microbiology · 2022
    Article
  6. Article
  7. Article
  8. Molecular characteristic ofVeterinary world · 2020
    Article
  9. Veterinary world · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ashraf M AbbasGenetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
Dalia A M Abd El-MoatyGenetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
Eman S A ZakiAerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
Elham F El-SerganyAnaerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
Nadine A El-SebayGenetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
Hala A FadlGenetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute, Cairo Egypt.
A A SamyDepartment of Microbiology and Immunology, Veterinary Division, National Research Center, Dokki, Egypt.
Veterinary Serum and Vaccine Research Institute · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimThis study aimed to create rapid characterization and genotyping of MATERIALS AND

methodsThirty bacterial isolates were characterized by capsular and somatic identification using conventional procedure followed by multiplex polymerase chain reaction (PCR), restriction endonucleases analysis (REA), and finally confirmed by sequence analysis. Two local vaccine strains and two field isolates were identified as PM Type A and B.

resultsA total of 30 isolates were found positive for PM either morphologically and biochemically; however, multiplex PCR technique identified only 22 isolates as

conclusionPCR and REA could confirm the identity of PM and provide a rapid and reliable characterization in comparison with biochemical analysis and conventional serotyping that may take up to 2 weeks. Hence, they can reduce the time needed for polyvalent vaccine production and when the reference antisera are unavailable. Moreover, the identity of Omp-H for vaccine and field strains may provide better data to control Pasteurellosis in Egypt.

Indexed as

multiplex polymerase chain reactionouter membrane protein HPasteurella multocidarestriction endonucleases analysis

Identifiers

PMID30147273
PMCPMC6097567
OpenAlexW2883594868

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.