ArticleCancer letters2018
Modeling Wnt signaling by CRISPR-Cas9 genome editing recapitulates neoplasia in human Barrett epithelial organoids.
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Article in Cancer letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Harnessing human tumor organoids for cancer modeling and precision therapy.Protein & cell · 2026Review
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- Genetically Defined Organoid Models Reveal Mechanisms Driving Squamous Cell Neoplastic Evolution and Identify Potential Therapeutic Vulnerabilities.bioRxiv : the preprint server for biology · 2025Article
- Biomedical applications of organoids derived from the digestive system.Frontiers in cell and developmental biology · 2025Review
- Modeling neurodegenerative diseases with brain organoids: from development to disease applications.Frontiers in cell and developmental biology · 2025Review
- Engineering Heterogeneous Tumor Models for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Establishing mouse and human oral esophageal organoids to investigate the tumor immune response.Disease models & mechanisms · 2024Article
- Esophageal adenocarcinoma models: a closer look.Frontiers in molecular biosciences · 2024Review
- Modelling esophageal adenocarcinoma and Barrett's esophagus with patient-derived organoids.Frontiers in molecular biosciences · 2024Review
- Experimental models for cancer brain metastasis.Cancer pathogenesis and therapy · 2024Review
- Esophageal organoids: applications and future prospects.Journal of molecular medicine (Berlin, Germany) · 2023Review
- Engineered organoids in oral and maxillofacial regeneration.iScience · 2023Review
- Editorial: Reviews in molecular and cellular oncology.Frontiers in oncology · 2023Article
- The pivotal application of patient-derived organoid biobanks for personalized treatment of gastrointestinal cancers.Biomarker research · 2022Review
- A human Barrett's esophagus organoid system reveals epithelial-mesenchymal plasticity induced by acid and bile salts.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Investigation of early neoplastic transformation and premalignant biology using genetically engineered organoid models.Computational and structural biotechnology journal · 2022Review
- Cancer Risk and Mutational Patterns Following Organ Transplantation.Frontiers in cell and developmental biology · 2022Review
- A new murine esophageal organoid culture method and organoid-based model of esophageal squamous cell neoplasia.iScience · 2021Article
- Review
- Understanding the cellular origin and progression of esophageal cancer using esophageal organoids.Cancer letters · 2021Review
Corrections and comments
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Authors and funding
16 authors at 4 institutions in 2 countries.
Xi LiuDepartment of Pathology, The First Affiliated Hospital of Xi' an Jiaotong University, No. 277 Yanta West Road, Xi' an, 710061, Shaanxi, China; Division of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Yulan ChengDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
John M AbrahamDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Zhixiong WangDepartment of Gastrointestinal Surgery, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Zhe WangDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Xiquan KeDepartment of Gastroenterology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China.
Rong YanDepartment of Surgical Oncology, First Affiliated Hospital of Xi' an Jiaotong University, No. 277 Yanta West Road, Xi' an, 710061, Shaanxi, China.
Eun Ji ShinDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Saowanee NgamruengphongDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Mouen A KhashabDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Guanjun ZhangDepartment of Pathology, The First Affiliated Hospital of Xi' an Jiaotong University, No. 277 Yanta West Road, Xi' an, 710061, Shaanxi, China.
George McNamaraDivision of Gastroenterology - Ross Fluorescence Imaging Center, Department of Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA.
Andrew J EwaldDepartment of Cell Biology and Oncology, Center for Cell Dynamics, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
DeChen LinDivision of Hematology and Oncology, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA, 90048, USA.
Zhengwen LiuDepartment of Infectious Diseases, First Affiliated Hospital of Xi' an Jiaotong University, No. 277 Yanta West Road, Xi' an, 710061, Shaanxi, China.
Stephen J MeltzerDivision of Gastroenterology, Department of Medicine, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA; Division of Gastroenterology, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, School of Medicine, Baltimore, MD, 21205, USA. Electronic address: smeltzer@jhmi.edu.
Johns Hopkins University · USFirst Affiliated Hospital of Xi'an Jiaotong University · CNCedars-Sinai Medical Center · USFirst Affiliated Hospital of Bengbu Medical College · CN
Funding
Translational Research Enhancement CoreP30DK089502 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI ZACHOS, NICHOLAS CONSTANTINE · 2011 to 2020
$12.0MThe Role of Physical Cues in Collective Cell InvasionU54CA210173 · NCI · JOHNS HOPKINS UNIVERSITY · PI WIRTZ, DENIS · 2016 to 2020
$9.9MAcademic-Industrial Partnership for Non-invasive Barrett's Esophagus DetectionR01DK118250 · NIDDK · JOHNS HOPKINS UNIVERSITY · PI MELTZER, STEPHEN J · 2018 to 2022
$3.8MFacile screening for esophageal cancer in LMICsUH3CA211457 · NCI · JOHNS HOPKINS UNIVERSITY · PI MELTZER, STEPHEN J, WANG, TZA-HUEI JEFF · 2019 to 2021
$2.8M(PQC-1) Driver Events In IBD-Associated Neoplastic ProgressionR01CA190040 · NCI · JOHNS HOPKINS UNIVERSITY · PI MELTZER, STEPHEN J, SELARU, FLORIN · 2014 to 2017
$2.5MFacile screening for esophageal cancer in LMICsUG3CA211457 · NCI · JOHNS HOPKINS UNIVERSITY · PI MELTZER, STEPHEN J, WANG, TZA-HUEI JEFF · 2017 to 2018
$861kNCI NIH HHS R01 CA190040NCI NIH HHS U54 CA210173NCI NIH HHS UG3 CA211457NCI NIH HHS UH3 CA211457NIDDK NIH HHS P30 DK089502NIDDK NIH HHS R01 DK118250
Abstract
Primary organoid cultures generated from patient biopsies comprise a novel improved platform for disease modeling, being genetically stable and closely recapitulating in vivo scenarios. Barrett esophagus (BE) is the major risk factor for esophageal adenocarcinoma. There has been a dearth of long-term in vitro expansion models of BE neoplastic transformation. We generated a long-term virus-free organoid expansion model of BE neoplasia from patient biopsies. Both wild-type and paired APC-knockout (APC
Indexed as
CRISPR-Cas SystemsAdenomatous Polyposis Coli ProteinApoptosisBarrett Esophagusbeta CateninCell DifferentiationCell MovementCell ProliferationCell Transformation, NeoplasticGene EditingGene Knockout TechniquesHumansModels, GeneticOrganoidsWnt Signaling PathwayAdenomatous Polyposis Coli Proteinbeta CateninCTNNB1 protein, humanBarrett esophagusCRISPR/Cas9Neoplastic transformationOrganoidsWnt signaling
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.