Evidence map›Paper›PMID 30144507›Full record

ArticleMechanisms of development2018

Gain-of-function Shh mutants activate Smo cell-autonomously independent of Ptch1/2 function.

Catalina Casillas, Henk Roelink

Abstract read
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In one paragraph

Article in Mechanisms of development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. A Highly ConservedFrontiers in cell and developmental biology · 2021
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Catalina CasillasDepartment of Molecular and Cell Biology, 16 Barker Hall, 3204, University of California, Berkeley, CA 94720, USA.
Henk RoelinkDepartment of Molecular and Cell Biology, 16 Barker Hall, 3204, University of California, Berkeley, CA 94720, USA. Electronic address: roelink@berkeley.edu.

Funding

Sonic Hedgehog is a Calcium-regulated Zinc peptidaseR01GM117090 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI ROELINK, HENK · 2016 to 2022
$1.9M
NIGMS NIH HHS R01 GM117090
6 · The paper itself

Abstract

Sonic Hedgehog (Shh) signaling is characterized by non-cell autonomy; cells expressing Shh do not respond to the ligand. Here, we identify several Shh mutations that can activate the Hedgehog (Hh) pathway cell-autonomously. Cell-autonomous pathway activation requires the extracellular cysteine rich domain of Smoothened, but is otherwise independent of the Shh receptors Patched1 and -2. Many of the Shh mutants that gain activity fail to undergo auto processing resulting in the perdurance of the Shh pro-peptide, a form of Shh that is sufficient to activate the Hh response cell-autonomously. Our results demonstrate that Shh is capable of activating the Hh pathway via Smoothened, independently of Patched1/2, and that it harbors an intrinsic mechanism that prevents cell-autonomous activation of the Shh response.

Indexed as

Amino Acid SequenceAnimalsChickensGain of Function MutationHedgehog ProteinsHoloprosencephalyLuciferasesMiceModels, BiologicalNeural TubePatched-1 ReceptorPatched-2 ReceptorProtein DomainsSmoothened ReceptorZinc Finger Protein GLI1Hedgehog ProteinsLuciferasesPatched-1 ReceptorPatched-2 ReceptorPtch1 protein, mousePtch2 protein, mouseShh protein, mouseSmoothened ReceptorSmo protein, mouseZinc Finger Protein GLI1

Identifiers

PMID30144507
PMCPMC6165682

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.