Evidence map›Paper›PMID 30144444›Full record

ArticleExperimental cell research2018

Distinct mechanisms enable inward or outward budding from late endosomes/multivesicular bodies.

Monica Gireud-Goss, Sahily Reyes, Marenda Wilson, Madeline Farley, Kimiya Memarzadeh, Saipraveen Srinivasan, Natalie Sirisaengtaksin, Shinji Yamashita, Susan Tsunoda, Frederick F Lang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Monica Gireud-GossDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Monica.B.Gireud@uth.tmc.edu.
Sahily ReyesDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Sahily.Reyes@uth.tmc.edu.
Marenda WilsonThe University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Marenda.A.Wilson@uth.tmc.edu.
Madeline FarleyDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Madeline.M.Burgoyne@uth.tmc.edu.
Kimiya MemarzadehDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Kimiya.Memarzadeh@uth.tmc.edu.
Saipraveen SrinivasanUT Department of Cell Biology, Southwestern Medical Center, Dallas, TX 75235, USA. Electronic address: Saipraveen.Srinivasan@UTSouthwestern.edu.
Natalie SirisaengtaksinDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA. Electronic address: Natalie.Sirisaengtaksin@uth.tmc.edu.
Shinji YamashitaDepartment of Neurosurgery, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA. Electronic address: shinjy@med.miyazaki-u.ac.jp.
Susan TsunodaDepartment of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA. Electronic address: susan.tsunoda@colostate.edu.
Frederick F LangDepartment of Neurosurgery, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA. Electronic address: flang@mdanderson.org.
M Neal WaxhamDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA. Electronic address: M.N.Waxham@uth.tmc.edu.
Andrew J BeanDepartment of Neurobiology and Anatomy, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; Biochemistry and Cell Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA; The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA; Department of Pediatrics, The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA. Electronic address: A.Bean@uth.tmc.edu.

Funding

EARLY STEPS IN RECEPTOR-MEDIATED ENDOCYTOSISR01GM042455 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI METTLEN, MARCEL BERNARD · 1989 to 2020
$8.2M
MOLECULAR MECHANISMS OF NEUROTRANSMITTER SECRETIONR01MH058920 · NIMH · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI BEAN, ANDREW J · 1998 to 2010
$2.9M
The Role of Growth Factor Receptor Trafficking in TumorigenesisR01CA166749 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ZAGE, PETER E. · 2013 to 2017
$1.7M
NCI NIH HHS R01 CA166749NIGMS NIH HHS R01 GM042455NIMH NIH HHS R01 MH058920
6 · The paper itself

Abstract

Regulating the residence time of membrane proteins on the cell surface can modify their response to extracellular cues and allow for cellular adaptation in response to changing environmental conditions. The fate of membrane proteins that are internalized from the plasma membrane and arrive at the limiting membrane of the late endosome/multivesicular body (MVB) is dictated by whether they remain on the limiting membrane, bud into internal MVB vesicles, or bud outwardly from the membrane. The molecular details underlying the disposition of membrane proteins that transit this pathway and the mechanisms regulating these trafficking events are unclear. We established a cell-free system that reconstitutes budding of membrane protein cargo into internal MVB vesicles and onto vesicles that bud outwardly from the MVB membrane. Both budding reactions are cytosol-dependent and supported by Saccharomyces cerevisiae (yeast) cytosol. We observed that inward and outward budding from the MVB membrane are mechanistically distinct but may be linked, such that inhibition of inward budding triggers a re-routing of cargo from inward to outward budding vesicles, without affecting the number of vesicles that bud outwardly from MVBs.

Indexed as

Adaptor Proteins, Signal TransducingCell-Free SystemCell MembraneEndosomal Sorting Complexes Required for TransportGene Expression RegulationHeLa CellsHumansIntracellular MembranesLysosomesMultivesicular BodiesPhosphoproteinsProtein TransportSaccharomyces cerevisiaeSignal TransductionAdaptor Proteins, Signal TransducingEndosomal Sorting Complexes Required for Transporthepatocyte growth factor-regulated tyrosine kinase substratePhosphoproteinsSTAM protein, humanBuddingCell-free assayDynaminEndosomeESCRTMultivesicular bodyRecycling

Identifiers

PMID30144444
PMCPMC7444440

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.