ArticleBiotechnology and bioengineering2018
Neonatal testis growth recreated in vitro by two-dimensional organ spreading.
Article in Biotechnology and bioengineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
- Generation of Human Bioartificial Tissues Using Agarose-Derived Biomaterials.Materials (Basel, Switzerland) · 2026Review
- An adverse outcome pathway-aligned ex vivo mouse testicular organ culture platform for mechanistic integration of multi-level endpoints with recovery assessment.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.PloS one · 2026Article
- Agarose Gel-Supported Culture of Cryopreserved Calf Testicular Tissues.Veterinary sciences · 2025Article
- 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.Advanced healthcare materials · 2025Article
- Development of the membrane ceiling method for in vitro spermatogenesis.Scientific reports · 2025Article
- A long-term mouse testis organ culture system to identify germ cell damage induced by chemotherapy.Current research in toxicology · 2025Article
- Synthesis, Anti-Cancer Activity, Cell Cycle Arrest, Apoptosis Induction, and Docking Study of Fused Benzo[Molecules (Basel, Switzerland) · 2024Article
- Application of testicular organ culture system for the evaluation of spermatogenesis impairment.Scientific reports · 2024Article
- Alkylating agents-induced gonadotoxicity in prepubertal males: Insights on the clinical and preclinical front.Clinical and translational science · 2024Review
- Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control.The Journal of reproduction and development · 2024Article
- Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.Scientific reports · 2023Article
- Generation of rat offspring using spermatids produced through in vitro spermatogenesis.Scientific reports · 2023Article
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Complete meiosis in rat prepubertal testicular tissue under in vitro sequential culture conditions.Andrology · 2023Article
- Article
- Review
- Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source.Animals : an open access journal from MDPI · 2022Article
- Biomaterial strategies for the application of reproductive tissue engineering.Bioactive materials · 2022Review
- Quantitative understanding of HepaRG cells during drug-induced intrahepatic cholestasis through changes in bile canaliculi dynamics.Pharmacology research & perspectives · 2022Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organ culture experiments can be hampered by central degeneration or necrosis due to the inadequate permeation of oxygen and nutrients, which deteriorates the function and growth of cultured tissues. In the current study, we aimed to overcome this limitation of organ culture through spreading the tissue two dimensionally on an agarose gel stand and molding into a disc shape by placing a ceiling of polydimethylsiloxane (PDMS) chip, which is highly oxygen permeable. By this, every part of the tissue can receive a sufficient supply of oxygen through PDMS as well as nutrients through the agarose gel below. This method not only prevented central necrosis of tissues, but also supported the tissue growth over time. In addition, such growth, as volume enlargement, could be easily measured. Under these conditions, we examined the effect of several factors on the growth of neonatal mouse testis, and found that follicle stimulating hormone (FSH) and insulin significantly promoted the growth. These results are in good agreement with previous in vivo reports. Notably, the growth achieved over 7 days in our in vitro system is almost comparable to, about 80% of, that observed in vivo. Thus, we successfully monitored the promotion of tissue growth beyond the limits of the conventional organ culture method. This extremely simple method could offer a unique platform to evaluate the growth as well as functional properties of organs, not only the testis but also others as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.