ArticleScientific reports2018
Direct production of a genetically-encoded immobilized biodiesel catalyst.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 45 citations in OpenAlex.
- Review
- High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes.Applied microbiology and biotechnology · 2024Review
- Magnetic protein aggregates generated by supramolecular assembly of ferritin cages - a modular strategy for the immobilization of enzymes.Frontiers in bioengineering and biotechnology · 2024Article
- A Bioresponsive Genetically Encoded Antimicrobial Crystal for the Oral Treatment of Helicobacter Pylori Infection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Cell-free protein crystallization for nanocrystal structure determination.Scientific reports · 2022Article
- Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme.ACS synthetic biology · 2022Article
- Can (We Make)Toxins · 2021Review
- Structure and Dynamics of an Archeal Monoglyceride Lipase fromBiomolecules · 2021Article
- Production of Cross-Linked Lipase Crystals at a Preparative Scale.Crystal growth & design · 2021Article
- Catalytically-active inclusion bodies for biotechnology-general concepts, optimization, and application.Applied microbiology and biotechnology · 2020Review
- Targeted Myoglobin Delivery as a Strategy for Enhancing the Sensitivity of Hypoxic Cancer Cells to Radiation.iScience · 2020Article
- Chemical treatments for modification and immobilization to improve the solvent-stability of lipase.World journal of microbiology & biotechnology · 2019Review
- Tailoring the surface area and the acid-base properties of ZrOScientific reports · 2019Article
- Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.Biotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of immobilized enzymes as biocatalysts has great potential to improve the efficiency and environmental sustainability of many industrial processes. Here, we report a novel approach that allows for the direct production of a highly active immobilized lipase within the bacterium Bacillus thuringiensis. Cry3Aa-lipA crystals were generated by genetically fusing Bacillus subtilis lipase A to Cry3Aa, a protein that naturally forms crystals in the bacteria. The crystal framework significantly stabilized the lipase against denaturation in organic solvents and high temperatures, resulting in a highly efficient fusion crystal that could catalyze the conversion of triacylglycerols to fatty acid methyl ester biodiesel to near-completion over 10 cycles. The simplicity and robustness of the Cry-fusion crystal (CFC) immobilization system could make it an appealing platform for generating industrial biocatalysts for multiple bioprocesses.
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