Evidence map›Paper›PMID 30143683›Full record

ArticleScientific reports2018

From individual to collective 3D cancer dissemination: roles of collagen concentration and TGF-β.

J Plou, Y Juste-Lanas, V Olivares, C Del Amo, C Borau, J M García-Aznar

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
2.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 85 citations in OpenAlex.

  1. Review
  2. Microphysiological systems for metastasis research: a stepwise approach.Cellular oncology (Dordrecht, Netherlands) · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

J PlouMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain. jplou@cicbiomagune.es.
Y Juste-LanasMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain.
V OlivaresMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain.
C Del AmoMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain.
C BorauMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain.
J M García-AznarMultiscale in Mechanical and Biological Engineering, Aragon Institute of Engineering Research (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018, Zaragoza, Spain. jmgaraz@unizar.es.ORCID 0000-0002-9864-7683
Universidad de Zaragoza · ES

Funding

European Research Council 306571
6 · The paper itself

Abstract

Cancer cells have the ability to migrate from the primary (original) site to other places in the body. The extracellular matrix affects cancer cell migratory capacity and has been correlated with tissue-specific spreading patterns. However, how the matrix orchestrates these behaviors remains unclear. Here, we investigated how both higher collagen concentrations and TGF-β regulate the formation of H1299 cell (a non-small cell lung cancer cell line) spheroids within 3D collagen-based matrices and promote cancer cell invasive capacity. We show that at low collagen concentrations, tumor cells move individually and have moderate invasive capacity, whereas when the collagen concentration is increased, the formation of cell clusters is promoted. In addition, when the concentration of TGF-β in the microenvironment is lower, most of the clusters are aggregates of cancer cells with a spheroid-like morphology and poor migratory capacity. In contrast, higher concentrations of TGF-β induced the formation of clusters with a notably higher invasive capacity, resulting in clear strand-like collective cell migration. Our results show that the concentration of the extracellular matrix is a key regulator of the formation of tumor clusters that affects their development and growth. In addition, chemical factors create a microenvironment that promotes the transformation of idle tumor clusters into very active, invasive tumor structures. These results collectively demonstrate the relevant regulatory role of the mechano-chemical microenvironment in leading the preferential metastasis of tumor cells to specific tissues with high collagen concentrations and TFG-β activity.

Indexed as

Imaging, Three-DimensionalActinsAnimalsCattleCell Line, TumorCell MovementCell ProliferationCell ShapeCollagenHumansHydrogel, Polyethylene Glycol DimethacrylateMicrofluidicsMultivariate AnalysisNeoplasmsPorositySpheroids, CellularActinsCollagenHydrogel, Polyethylene Glycol DimethacrylateTransforming Growth Factor beta

Identifiers

PMID30143683
PMCPMC6109049
OpenAlexW2888232470

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.