ArticleNature communications2018
Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Spectral phasor imaging on a commercial confocal microscope without a spectral detector.Scientific reports · 2025Article
- Visualizing Epigenetics: A Review of Microscopy Techniques for Investigating DNA Methylation Patterns, Chromatin Structure, and Gene Expression.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2025Review
- Exploiting the detector distance information in image scanning microscopy by phasor-based SPLIT-ISM.Biomedical optics express · 2025Article
- Super-resolved analysis of colocalization between replication and transcription along the cell cycle in a model of oncogene activation.Communications biology · 2024Article
- Facile construction of dual-response super-resolution probes for tracking organelles dynamics.Exploration (Beijing, China) · 2024Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Multiplexed imaging in live cells using pulsed interleaved excitation spectral FLIM.Optics express · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Phasor plots and the future of spectral and lifetime imaging.Nature methods · 2023Article
- Spatial resolution enhancement in photon-starved STED imaging using deep learning-based fluorescence lifetime analysis.Nanoscale · 2023Article
- SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole.Scientific reports · 2023Article
- Alterations induced by the PML-RARα oncogene revealed by image cross correlation spectroscopy.Biophysical journal · 2022Article
- A phasor-based approach to improve optical sectioning in any confocal microscope with a tunable pinhole.Microscopy research and technique · 2022Article
- Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS).Scientific reports · 2021Article
- Super-Resolution Imaging with Graphene.Biosensors · 2021Review
- Phasor approach of Mueller matrix optical scanning microscopy for biological tissue imaging.Biophysical journal · 2021Article
- Single-cell imaging of genome organization and dynamics.Molecular systems biology · 2021Review
- Chromatin investigation in the nucleus using a phasor approach to structured illumination microscopy.Biophysical journal · 2021Article
- Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.