ArticleBMC oral health2018
Characterization of oral polymorphonuclear neutrophils in periodontitis patients: a case-control study.
Article in BMC oral health, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 75 citations in OpenAlex.
- CD11b as a diagnostic biomarker of rheumatoid arthritis and as a risk factor of periodontitis: a pilot study.Odontology · 2026Observational
- Mucosal Inflammation Shapes Human Neutrophil States in Tissue and Circulation.bioRxiv : the preprint server for biology · 2026Article
- Distinct functional profiles of oral neutrophils in molar incisor pattern periodontitis, generalized periodontitis and periodontal health.Scientific reports · 2026Article
- Functions of Macrophages in Periodontal Disease Progression and Gingival Tissue Homeostasis.Advances in experimental medicine and biology · 2026Review
- Review
- The Role of Reactive Oxygen Species (ROS) in Periodontitis: A Potential Therapeutic Target.Immunity, inflammation and disease · 2025Review
- Article
- E-Cigarette effects on oral health: A molecular perspective.Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2025Review
- Periodontal diseases in Down syndrome during childhood: a scoping review.BMC oral health · 2025Article
- The impact of aging on neutrophil functions and the contribution to periodontitis.International journal of oral science · 2025Review
- Methylome and transcriptome analyses reveal HLA-DMB's contribution to periodontitis development.PloS one · 2025Article
- Toward Digital Periodontal Health: Recent Advances and Future Perspectives.Bioengineering (Basel, Switzerland) · 2024Review
- Article
- Salivary bactericidal/permeability-increasing protein: A novel inflammatory marker associated with periodontitis.Clinical oral investigations · 2023Article
- Electroactive Biomaterials for Facilitating Bone Defect Repair under Pathological Conditions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Innovative biomaterials for the treatment of periodontal disease.Frontiers in dental medicine · 2023Review
- Uncovering the potential role of oxidative stress in the development of periodontitis and establishing a stable diagnostic model via combining single-cell and machine learning analysis.Frontiers in immunology · 2023Article
- Increase in the level of oral neutrophils with gingival inflammation - A population survey.The Saudi dental journal · 2022Article
- Review
- MMP-9 Levels in the Gingival Crevicular Fluid of Chilean Rosacea Patients.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMaintaining oral health is a continuous and dynamic process that also involves the immune system. Polymorphonuclear neutrophils (PMNs) migrate from blood circulation and become apparent in the oral fluid. Controversies exist regarding the specific role of the oral PMNs (oPMNs) in the presence of chronic oral inflammation, such as periodontitis. In this study we characterized cell counts, activation status, apoptosis, and reactive oxygen species (ROS) generation by oPMNs and circulatory (cPMNs), and the salivary protease activity, in subjects with and without periodontitis.
methodsVenous blood and oral rinse samples were obtained from 19 patients with untreated periodontitis and 16 control subjects for PMN isolation. Apoptosis and expression of cell activation markers CD11b, CD63, and CD66b were analyzed using flow cytometry. Constitutive ROS generation was detected using dihydrorhodamine123. Additionally, ROS production in response to stimulation was evaluated in samples incubated with 10 μM phorbol myristate acetate (PMA) or Fusobacterium nucleatum. Total protease activity was measured using substrate PEK-054.
resultsPeriodontitis patients presented with over 4 times higher oPMN counts compared to controls (p = 0.007), which was a predictor for the total protease activity (r
conclusionsHigher numbers of oral PMNs, being more apoptotic and having increased levels of degranulation markers were found in periodontitis compared to periodontal health. However, since the oPMNs in periodontitis were responsive to ex vivo stimulation, we conclude that the oPMNs are active in the oral ecosystem. It is currently unknown whether the oPMN counts, which correlated with the detected protease levels, are detrimental in the long term for the oral mucosa integrity.
trial registrationThis study was retrospectively registered at the ISRCTN registry (trial ID ISRCTN15252886 ). Registration date August 11, 2017.
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