Evidence map›Paper›PMID 30143034›Full record

ArticleGenome biology2018

Interaction between the microbiome and TP53 in human lung cancer.

K Leigh Greathouse, James R White, Ashely J Vargas, Valery V Bliskovsky, Jessica A Beck, Natalia von Muhlinen, Eric C Polley, Elise D Bowman, Mohammed A Khan, Ana I Robles and 23 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 297 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
297citing papers in PubMed, 3 pooled it
11.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

297 citing papers in PubMed, 3 syntheses or guidelines pooled it, 450 citations in OpenAlex.

  1. Pooled it
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  12. Harnessing the microbiome for cancer therapy.Nature reviews. Microbiology · 2026
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237 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

33 authors at 10 institutions in 1 country.

K Leigh GreathouseLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
James R WhiteResphera Biosciences, Baltimore, MD, 21231, USA.
Ashely J VargasLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Valery V BliskovskyCenter for Cancer Research Genomics Core, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Jessica A BeckLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Natalia von MuhlinenLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Eric C PolleyDivision of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, MN, 55905, USA.
Elise D BowmanLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Mohammed A KhanLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Ana I RoblesLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Tomer CooksLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Bríd M RyanLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA.
Noah PadgettDepartment of Educational Psychology, Baylor University, Waco, TX, 97346, USA.
Amiran H DzutsevLaboratory of Experimental Immunology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Giorgio TrinchieriLaboratory of Experimental Immunology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Marbin A PinedaGenetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health Bethesda, Bethesda, MD, 20892, USA.
Sven BilkeGenetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health Bethesda, Bethesda, MD, 20892, USA.
Paul S MeltzerGenetics Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health Bethesda, Bethesda, MD, 20892, USA.
Alexis N HokenstadDepartment of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA.
Tricia M StickrodMicrobiome Laboratory, Mayo Clinic, Rochester, MN, 55905, USA.
Marina R Walther-AntonioDepartment of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA.
Joshua P EarlDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Joshua C MellDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Jaroslaw E KrolDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Sergey V BalashovDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Archana S BhatDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Garth D EhrlichDepartment of Microbiology and Immunology, Center for Genomic Sciences, Institute of Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, PA, 19129, USA.
Alex ValmNational Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Clayton DemingNational Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Sean ConlanNational Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Julia OhJackson Laboratory, Framingham, CT, 06032, USA.
Julie A SegreNational Human Genome Research Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Curtis C HarrisLaboratory of Human Carcinogenesis, Center for Cancer, Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr., Rm 3068A, MSC 4258, Bethesda, MD, 20892-4258, USA. curtis_harris@nih.gov.
National Cancer Institute · USDrexel University · USCenter for Cancer Research · USNational Institutes of Health · USMayo Clinic in Arizona · USNational Human Genome Research Institute · USBaylor University · USJackson Laboratory · USMayo Clinic · USResphera Biosciences · US

Funding

Shared Resource ManagementP30CA034196 · NCI · JACKSON LABORATORY · PI William Carl Skarnes · 1985 to 2026
$61.9M
ZESTRIL VS. VERAPAMIL IN THE TREATMENT OF HYPERTENSIONM01RR000585 · NCRR · MAYO CLINIC ROCHESTER · PI RIZZA, ROBERT A. · 1985 to 2006
$30.4M
Gene-Environment Interactions at the Skin SurfaceZIAHG000180 · NHGRI · NATIONAL HUMAN GENOME RESEARCH INSTITUTE · PI SEGRE, JULIA ANGELA · 2009 to 2025
$29.6M
Institutional Career Development CoreKL2TR002379 · NCATS · MAYO CLINIC ROCHESTER · PI NILUFER ERTEKIN-TANER · 2017 to 2026
$14.9M
NCATS NIH HHS KL2 TR002379NCI NIH HHS IntramuralNCI NIH HHS P30 CA034196NCRR NIH HHS M01 RR000585
6 · The paper itself

Abstract

backgroundLung cancer is the leading cancer diagnosis worldwide and the number one cause of cancer deaths. Exposure to cigarette smoke, the primary risk factor in lung cancer, reduces epithelial barrier integrity and increases susceptibility to infections. Herein, we hypothesize that somatic mutations together with cigarette smoke generate a dysbiotic microbiota that is associated with lung carcinogenesis. Using lung tissue from 33 controls and 143 cancer cases, we conduct 16S ribosomal RNA (rRNA) bacterial gene sequencing, with RNA-sequencing data from lung cancer cases in The Cancer Genome Atlas serving as the validation cohort.

resultsOverall, we demonstrate a lower alpha diversity in normal lung as compared to non-tumor adjacent or tumor tissue. In squamous cell carcinoma specifically, a separate group of taxa are identified, in which Acidovorax is enriched in smokers. Acidovorax temporans is identified within tumor sections by fluorescent in situ hybridization and confirmed by two separate 16S rRNA strategies. Further, these taxa, including Acidovorax, exhibit higher abundance among the subset of squamous cell carcinoma cases with TP53 mutations, an association not seen in adenocarcinomas.

conclusionsThe results of this comprehensive study show both microbiome-gene and microbiome-exposure interactions in squamous cell carcinoma lung cancer tissue. Specifically, tumors harboring TP53 mutations, which can impair epithelial function, have a unique bacterial consortium that is higher in relative abundance in smoking-associated tumors of this type. Given the significant need for clinical diagnostic tools in lung cancer, this study may provide novel biomarkers for early detection.

Indexed as

AdultAgedBiodiversityComamonadaceaeFemaleHumansLung NeoplasmsMaleMicrobiotaMiddle AgedMutationNeoplasms, Squamous CellProteobacteriaReproducibility of ResultsSmokersTumor Suppressor Protein p53Tumor Suppressor Protein p53Lung cancerMicrobiomeMutationSquamous cell carcinomaTP53

Identifiers

PMID30143034
PMCPMC6109311
OpenAlexW2888696120

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.