Evidence map›Paper›PMID 30141131›Full record

ArticleArchives of virology2018

A fast and simple one-step duplex PCR assay for canine distemper virus (CDV) and canine coronavirus (CCoV) detection.

Jing Wang, Yakun Luo, Lin Liang, Jinxiang Li, Shangjin Cui

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Archives of virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Jing WangInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China.
Yakun LuoInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China.
Lin LiangInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China.
Jinxiang LiInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China. lijinxiang@caas.cn.
Shangjin CuiInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road 2, Haidian District, Beijing, 100193, China. cuishangjin@caas.cn.
Chinese Academy of Agricultural Sciences · CNInstitute of Animal Sciences · CN

Funding

Agricultural Science and Technology Innovation Program ASTIP-IAS15National Key Research and Development Program of China 2016YFD0501003National Key Research and Development Program of China 2017YFD0501603National Key Research and Development Program of China 2017YFD0502300
6 · The paper itself

Abstract

The one-step polymerase chain reaction (one-step PCR) detection assay is an innovative PCR detection method, eliminating nucleic acid extraction steps, in which samples can be directly added to PCR reagents for testing. For simultaneous detection of CDV and CCoV, a sensitive and specific one-step duplex PCR (one-step dPCR) assay was developed with two pairs of primers that were designed based on H and M gene sequences of CDV and CCoV, respectively. The one-step dPCR with optimized detection conditions has high specificity and sensitivity; independent sequencing assays further verified these results.

Indexed as

AnimalsCoronavirus, CanineCoronavirus InfectionsDistemperDistemper Virus, CanineDNA PrimersDogsPolymerase Chain ReactionRNA, ViralSensitivity and SpecificityDNA PrimersRNA, Viral

Identifiers

PMID30141131
PMCPMC7087121
OpenAlexW2888291836

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.