Evidence map›Paper›PMID 30141050›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice.

Matthew J Stroud, Xi Fang, Jennifer Veevers, Ju Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 80% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Matthew J StroudDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA.
Xi FangDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA.
Jennifer VeeversDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA.
Ju ChenDepartment of Medicine, University of California, San Diego, La Jolla, CA, USA. juchen@ucsd.edu.
University of California, San Diego · USKing's College London · GB

Funding

The Cardiac Role of Filamin CR01HL144872 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU · 2019 to 2022
$1.6M
ALPK3 in cardiac function and diseaseR01HL146759 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU · 2019 to 2022
$1.6M
BAG3 in Cardiac function and diseaseR01HL130295 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU · 2016 to 2019
$1.6M
Adipocytes and cardiac remodelingR01HL130452 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU · 2016 to 2019
$1.6M
The role of Nexilin in cardiomyocyte and cardiomyopathyR01HL137957 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU · 2017 to 2020
$1.6M
Luma in Cardiac Function and DiseaseR01HL123626 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, JU, EVANS, SYLVIA M · 2014 to 2017
$1.5M
British Heart Foundation FS/17/57/32934NHLBI NIH HHS R01 HL123626NHLBI NIH HHS R01 HL130295NHLBI NIH HHS R01 HL130452NHLBI NIH HHS R01 HL137957NHLBI NIH HHS R01 HL144872NHLBI NIH HHS R01 HL146759
6 · The paper itself

Abstract

The linker of nucleoskeleton and cytoskeleton (LINC) complex mediates intracellular cross talk between the nucleus and the cytoplasm. In striated muscle, the LINC complex provides structural support to the myocyte nucleus and plays an essential role in regulating gene expression and mechanotransduction. A wide range of cardiac and skeletal myopathies have been linked to mutations in LINC complex proteins. Studies utilizing tissue-specific knockout and mutant mouse models have revealed important insights into the roles of the LINC complex in striated muscle. In this chapter, we describe several feasible approaches for generating striated muscle-specific gene knockout and mutant mouse models to study LINC complex protein function in cardiac and skeletal muscle. The experimental procedures used for phenotyping and analysis of LINC complex knockout mice are also described.

Indexed as

MutationAllelesAmino Acid SubstitutionAnimalsBiomarkersCRISPR-Cas SystemsCytoskeletonEchocardiographyFluorescent Antibody TechniqueGene TargetingGenotypeMiceMice, KnockoutMice, TransgenicMultiprotein ComplexesMuscle, StriatedBiomarkersMultiprotein ComplexesNuclear ProteinsCardiac muscleCre/loxPCRISPR/Cas9Knock-in mouseKnockout mouseLINC complexNuclear envelopeSkeletal muscleStriated muscle

Identifiers

PMID30141050
PMCPMC6887482
OpenAlexW2888008003

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.