Evidence map›Paper›PMID 30140403›Full record

ArticleIranian journal of basic medical sciences2018

Spermatogenesis after transplantation of adipose tissue-derived mesenchymal stem cells in busulfan-induced azoospermic hamster.

Negar Karimaghai, Amin Tamadon, Farhad Rahmanifar, Davood Mehrabani, Alireza Raayat Jahromi, Shahrokh Zare, Zahra Khodabandeh, Iman Razeghian Jahromi, Omid Koohi-Hoseinabadi, Mehdi Dianatpour

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Iranian journal of basic medical sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Iranian journal of medical sciences · 2026
    Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Cell-Based Therapy Approaches in Treatment of Non-obstructive Azoospermia.Reproductive sciences (Thousand Oaks, Calif.) · 2023
    Review
  13. Review
  14. Article
  15. Review
  16. Is It Possible to Treat Infertility with Stem Cells?Reproductive sciences (Thousand Oaks, Calif.) · 2021
    Review
  17. Article
  18. Article
  19. Article
  20. Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Negar KarimaghaiStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Amin TamadonStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Farhad RahmanifarDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Davood MehrabaniStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Alireza Raayat JahromiDepartment of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Shahrokh ZareStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Zahra KhodabandehStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Iman Razeghian JahromiCardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Omid Koohi-HoseinabadiCentral Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran.
Mehdi DianatpourStem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz University of Medical Sciences · IRShiraz University · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesAdipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration of germinal layer cells of seminiferous tubules in busulfan-induced azoospermic hamsters. MATERIALS AND

methodsIn the present experimental case-control study, AT-MSCs were isolated from adipose tissue of two female and six male donor albino hamsters, and testes of the males were simultaneously used as negative control group. Six mature male recipient hamsters received two doses of busulfan with three weeks interval to stop endogenous spermatogenesis. Right testis of hamsters was intratubular injected with AT-MSCs via efferent duct 35 days after induction of azoospermia and was used as cell therapy group. The left testis without cell therapy was served as azoospermia group.

resultsAfter 35 days, testes and epididymis in all groups were removed for histological evaluation. Histomorphometric analyses of AT-MSCs-treated testes and epididymis showed that the epithelial tissue of seminiferous tubules was normally repaired in most cell-treated seminiferous tubules, and spermatozoa were present in epididymis tubes in comparison with intact testes. The untreated seminiferous tubules and epididymis tubes of azoospermia group were empty.

conclusionAllotransplanted AT-MSCs could successfully induce spermatogenesis in azoospermic seminiferous tubules of hamster. Therefore, AT-MSCs can be suggested as an attractive candidate in cell transplantation of azoospermia.

Indexed as

Adipose tissueAzoospermiaCell therapyHamsterMesenchymal stem cells

Identifiers

PMID30140403
PMCPMC6098960
OpenAlexW2884950438

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.