Evidence map›Paper›PMID 30139390›Full record

ArticleSkeletal muscle2018

Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line.

Yasuo Kitajima, Yusuke Ono

Open access · goldAbstract read
In one paragraph

Article in Skeletal muscle, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
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  6. Pax7 reporter mouse models: a pocket guide for satellite cell research.European journal of translational myology · 2023
    Article
  7. Article
  8. Article
  9. Transplantation to study satellite cell heterogeneity in skeletal muscle.Frontiers in cell and developmental biology · 2022
    Review
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  12. RNU (Bioengineering (Basel, Switzerland) · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Yasuo KitajimaMusculoskeletal Molecular Biology Research Group, Basic and Translational Research Center for Hard Tissue Disease, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan.
Yusuke OnoMusculoskeletal Molecular Biology Research Group, Basic and Translational Research Center for Hard Tissue Disease, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan. yusuke-ono@nagasaki-u.ac.jp.ORCID 0000-0002-4802-7507
Japan Agency for Medical Research and Development · JPJapan Society for the Promotion of Science · JP

Funding

Japan Agency for Medical Research and Development 16bm0704010h0001; 18ek0109383h0001Japan Society for the Promotion of Science 16J00431KAKENHI 15H05368, 16K13046, 18H03193 and 18K19749KAKENHI 15K16486
6 · The paper itself

Abstract

backgroundSatellite cells are residential muscle stem cells that express a paired box protein, PAX7.

resultsHere, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7

conclusionsTaken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases.

Indexed as

AnimalsCell DifferentiationCell ProliferationCells, CulturedGene Knock-In TechniquesGreen Fluorescent ProteinsMiceMice, Inbred C57BLPAX7 Transcription FactorSatellite Cells, Skeletal MuscleGreen Fluorescent ProteinsPax7 protein, mousePAX7 Transcription FactorKnock-in mouseMuscle regenerationMyogenesisPax7YFP

Identifiers

PMID30139390
PMCPMC6108100
OpenAlexW2888324245

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.