ArticleSkeletal muscle2018
A robust Pax7EGFP mouse that enables the visualization of dynamic behaviors of muscle stem cells.
Article in Skeletal muscle, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Integrating and Orchestrating Multiple Components of Skeletal Muscle Injury and Regeneration.American journal of physiology. Cell physiology · 2026Article
- TRF2 couples muscle stem cell identity to regenerative repair.Science advances · 2026Article
- Efficacy of neonatal mouse muscle extracellular vesicles in skeletal muscle repair and regeneration.Cell regeneration (London, England) · 2026Article
- Serum-free cultured meat production by usingiScience · 2025Article
- REST/NRSF Preserves muscle stem cell identity by repressing alternate cell fate.Nature communications · 2025Article
- 3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function.Advanced biology · 2025Article
- Beyond the bulk: overview and novel insights into the dynamics of muscle satellite cells during muscle regeneration.Inflammation and regeneration · 2024Review
- Pax7 reporter mouse models: a pocket guide for satellite cell research.European journal of translational myology · 2023Article
- Overlapping functions of SIX homeoproteins during embryonic myogenesis.PLoS genetics · 2023Article
- Generation of a MyoD knock-in reporter mouse line to study muscle stem cell dynamics and heterogeneity.iScience · 2023Article
- Machine learning-based classification of dual fluorescence signals reveals muscle stem cell fate transitions in response to regenerative niche factors.NPJ Regenerative medicine · 2023Article
- Piezo1 regulates the regenerative capacity of skeletal muscles via orchestration of stem cell morphological states.Science advances · 2022Article
- Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution.eLife · 2022Article
- Gli1 Defines a Subset of Fibro-adipogenic Progenitors that Promote Skeletal Muscle Regeneration With Less Fat Accumulation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening.Cell reports · 2021Article
- Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse.Skeletal muscle · 2021Article
- Perspectives on skeletal muscle stem cells.Nature communications · 2021Review
- Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation.Skeletal muscle · 2020Review
- Article
- Phosphorylation of TET2 by AMPK is indispensable in myogenic differentiation.Epigenetics & chromatin · 2019Article
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
backgroundPax7 is a transcription factor involved in the specification and maintenance of muscle stem cells (MuSCs). Upon injury, MuSCs leave their quiescent state, downregulate Pax7 and differentiate, contributing to skeletal muscle regeneration. In the majority of regeneration studies, MuSCs are isolated by fluorescence-activated sorting (FACS), based on cell surface markers. It is known that MuSCs are a heterogeneous population and only a small percentage of isolated cells are true stem cells that are able to self-renew. A strong Pax7 reporter line would be valuable to study the in vivo behavior of Pax7-expressing stem cells.
methodsWe generated and characterized the muscle properties of a new transgenic Pax7EGFP mouse. Utilizing traditional immunofluorescence assays, we analyzed whole embryos and muscle sections by fluorescence microscopy, in addition to whole skeletal muscles by 2-photon microscopy, to detect the specificity of EGFP expression. Skeletal muscles from Pax7EGFP mice were also evaluated in steady state and under injury conditions. Finally, MuSCs-derived from Pax7EGFP and control mice were sorted and analyzed by FACS and their myogenic activity was comparatively examined.
resultsOur studies provide a new Pax7 reporter line with robust EGFP expression, detectable by both flow cytometry and fluorescence microscopy. Pax7EGFP-derived MuSCs have identical properties to that of wild-type MuSCs, both in vitro and in vivo, excluding any positional effect due to the transgene insertion. Furthermore, we demonstrated high specificity of EGFP to label MuSCs in a temporal manner that recapitulates the reported Pax7 expression pattern. Interestingly, immunofluorescence analysis showed that the robust expression of EGFP marks cells in the satellite cell position of adult muscles in fixed and live tissues.
conclusionsThis mouse could be an invaluable tool for the study of a variety of questions related to MuSC biology, including but not limited to population heterogeneity, polarity, aging, regeneration, and motility, either by itself or in combination with mice harboring additional genetic alterations.
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