ArticleJournal of burn care & research : official publication of the American Burn Association2019
Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model.
Article in Journal of burn care & research : official publication of the American Burn Association, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Dimensional analysis as a tool for biofilm comparative analysis: bridging the interdisciplinary divides.Journal of bacteriology · 2026Review
- UnderstandingMicroorganisms · 2026Review
- Real-time detection and monitoring of bacteria in diabetic wounds using bacterial fluorescence.Frontiers in microbiology · 2026Article
- A research agenda for burn infection prevention: identifying knowledge gaps and prioritizing future directions.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2025Review
- Development of a Stringent Ex Vivo-Burned Porcine Skin Wound Model to Screen Topical Antimicrobial Agents.Antibiotics (Basel, Switzerland) · 2024Article
- The rat as an animal model in chronic wound research: An update.Life sciences · 2024Review
- Phylogenetic evaluation and genotypic identification of burn-related Pseudomonas aeruginosa strains isolated from post-burn human infections during hospitalization.Pathogens and disease · 2024Article
- Parallel Evolution of Pseudomonas aeruginosa during a Prolonged ICU-Infection Outbreak.Microbiology spectrum · 2022Article
- Catheter-associated urinary tract infection byProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comparative Transcriptome Analysis of Superficial and Deep Partial-Thickness Burn Wounds in Yorkshire vs Red Duroc Pigs.Journal of burn care & research : official publication of the American Burn Association · 2022Article
- Resistance Is Not Futile: The Role of Quorum Sensing Plasticity inMicroorganisms · 2022Review
- Histological assessment, anti-quorum sensing, and anti-biofilm activities of Dioon spinulosum extract: in vitro and in vivo approach.Scientific reports · 2022Article
- Biofilm model on mice skin wounds.Acta cirurgica brasileira · 2022Article
- Recent Advances in Experimental Burn Models.Biology · 2021Review
- Bacteriostatic Potential of Melatonin: Therapeutic Standing and Mechanistic Insights.Frontiers in immunology · 2021Review
- Cerium Nitrate Treatment Provides Eschar Stabilization through Reduction in Bioburden, DAMPs, and Inflammatory Cytokines in a Rat Scald Burn Model.Journal of burn care & research : official publication of the American Burn Association · 2020Article
- Identification of Metagenomics Structure and Function Associated With Temporal Changes in Rat (Rattus norvegicus) Skin Microbiome During Health and Cutaneous Burn.Journal of burn care & research : official publication of the American Burn Association · 2020Article
- Cerium nitrate enhances anti-bacterial effects and imparts anti-inflammatory properties to silver dressings in a rat scald burn model.International journal of burns and trauma · 2020Article
- Temporal shifts in the mycobiome structure and network architecture associated with a rat (Rattus norvegicus) deep partial-thickness cutaneous burn.Medical mycology · 2020Article
- Formation of Pseudomonas aeruginosa Biofilms in Full-thickness Scald Burn Wounds in Rats.Scientific reports · 2019Article
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Authors and funding
13 authors.
Funding
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Abstract
We used a modified Walker-Mason scald burn rat model to demonstrate that Pseudomonas aeruginosa, a common opportunistic pathogen in the burn ward and notable biofilm former, establishes biofilms within deep partial-thickness burn wounds in rats.Deep partial-thickness burn wounds, ~10% of the TBSA, were created in anesthetized male Sprague-Dawley rats (350-450 g; n = 84). Immediately post-burn, 100 µl of P. aeruginosa in phosphate-buffered saline at 1 × 103, 1 × 104, or 1 × 105 cells/wound was spread over the burn surface . At 1, 3, 7, and 11 days post-burn, animals were euthanized and blood and tissue were collected for complete blood counts, colony-forming unit (CFU) counts, biofilm gene expression, histology, scanning electron microscopy (SEM), and myeloperoxidase activity in the burn eschar.P. aeruginosa developed robust biofilm wound infections, plateauing at ~1 × 109 CFU/g burn tissue within 7 days regardless of inoculum size. Expression of Pseudomonas alginate genes and other virulence factors in the infected wound indicated formation of mature P. aeruginosa biofilm within the burn eschar. Compared to un-inoculated wounds, P. aeruginosa infection caused both local and systemic immune responses demonstrated by changes in systemic neutrophil counts, histology, and myeloperoxidase activity within the burn wound. Additionally, SEM showed P. aeruginosa enmeshed within an extracellular matrix on the burn surface as well as penetrating 500-600 µm deep into the eschar.P. aeruginosa establishes biofilms within deep partial-thickness burn wounds and invades deep into the burned tissue. This new in vivo biofilm infection model is valuable for testing novel anti-biofilm agents to advance burn care.
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