Evidence map›Paper›PMID 30135446›Full record

ArticleScientific reports2018

A platform for discovery of functional cell-penetrating peptides for efficient multi-cargo intracellular delivery.

Katrin Hoffmann, Nadia Milech, Suzy M Juraja, Paula T Cunningham, Shane R Stone, Richard W Francis, Mark Anastasas, Clinton M Hall, Tatjana Heinrich, Heique M Bogdawa and 20 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 62 citations in OpenAlex.

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  14. TargetedBiomedicines · 2021
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  18. Characterization and validation of a preventative therapy for hypertrophic cardiomyopathy in a murine model of the disease.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  19. In Vitro Assays: Friends or Foes of Cell-Penetrating Peptides.International journal of molecular sciences · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

30 authors at 5 institutions in 3 countries.

Katrin HoffmannTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Nadia MilechTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia. nadiam@phylogica.com.
Suzy M JurajaTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Paula T CunninghamTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Shane R StoneTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Richard W FrancisTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.ORCID 0000-0003-1580-7934
Mark AnastasasTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Clinton M HallTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Tatjana HeinrichTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Heique M BogdawaTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Scott WinslowTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Marie N ScobieTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Robert E DewhurstTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.ORCID 0000-0001-8441-4427
Laura FlorezTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Ferrer OngTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Maria KerfootTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Danie ChampainTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Abbie M AdamsCentre for Comparative Genomics, Murdoch University, Perth, WA, 6150, Australia.
Susan FletcherCentre for Comparative Genomics, Murdoch University, Perth, WA, 6150, Australia.ORCID 0000-0002-8632-641X
Helena M ViolaSchool of Human Sciences, The University of Western Australia, Crawley, WA, Australia.
Livia C HoolSchool of Human Sciences, The University of Western Australia, Crawley, WA, Australia.
Theresa ConnorTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Brooke A C LongvilleTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Yew-Foon TanTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Karen KroegerTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Volker MorathLehrstuhl für Biologische Chemie, Technische Universität München, 85354, Freising-Weihenstephan, Germany.ORCID 0000-0001-7636-0537
Gregory A WeissUniversity of California, Irvine, Department of Molecular Biology and Biochemistry, Irvine, CA, 92697-2025, USA.
Arne SkerraLehrstuhl für Biologische Chemie, Technische Universität München, 85354, Freising-Weihenstephan, Germany.ORCID 0000-0002-5717-498X
Richard M HopkinsTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
Paul M WattTelethon Kids Institute, University of Western Australia, Subiaco, Western Australia, Australia.
The Kids Research Institute Australia · AUMurdoch University · AUTechnical University of Munich · DEThe University of Western Australia · AUUniversity of California, Irvine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell penetrating peptides (CPPs) offer great potential to deliver therapeutic molecules to previously inaccessible intracellular targets. However, many CPPs are inefficient and often leave their attached cargo stranded in the cell's endosome. We report a versatile platform for the isolation of peptides delivering a wide range of cargos into the cytoplasm of cells. We used this screening platform to identify multiple "Phylomer" CPPs, derived from bacterial and viral genomes. These peptides are amenable to conventional sequence optimization and engineering approaches for cell targeting and half-life extension. We demonstrate potent, functional delivery of protein, peptide, and nucleic acid analog cargos into cells using Phylomer CPPs. We validate in vivo activity in the cytoplasm, through successful transport of an oligonucleotide therapeutic fused to a Phylomer CPP in a disease model for Duchenne's muscular dystrophy. This report thus establishes a discovery platform for identifying novel, functional CPPs to expand the delivery landscape of druggable intracellular targets for biological therapeutics.

Indexed as

AnimalsBacteriophage T7BiotinylationCarbon-Nitrogen LigasesCell-Penetrating PeptidesCHO CellsCircular DichroismCricetulusDisease Models, AnimalDrug Delivery SystemsDrug Evaluation, PreclinicalEscherichia coli ProteinsHEK293 CellsHumansMaleMice, Inbred C57BLbirA protein, E coliCarbon-Nitrogen LigasesCell-Penetrating PeptidesEscherichia coli ProteinsPeptide LibraryRepressor Proteins

Identifiers

PMID30135446
PMCPMC6105642
OpenAlexW2887744421

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.