ArticleBMC infectious diseases2018
Epidemiological analysis and rapid detection by one-step multiplex PCR assay of Haemophilus influenzae in children with respiratory tract infections in Zhejiang Province, China.
Article in BMC infectious diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 17 citations in OpenAlex.
- Simultaneous visual diagnosis of capsulated and non-capsulated Haemophilus influenzae using a duplex RPA method.Archives of microbiology · 2025Article
- Rapid and sensitive detection ofHeliyon · 2025Article
- Respiratory tract infections: an update on the complexity of bacterial diversity, therapeutic interventions and breakthroughs.Archives of microbiology · 2024Review
- Simultaneous Genetically Detection ofIranian journal of pathology · 2023Article
- Bacterial profiles and their antibiotic resistance background in superinfections caused by multidrug-resistant bacteria among COVID-19 ICU patients from southwest Iran.Journal of medical virology · 2023Article
- Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies.Infection and drug resistance · 2023Review
- Bronchial epithelia from adults and children: SARS-CoV-2 spread via syncytia formation and type III interferon infectivity restriction.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Establishment and Clinical Application of a RPA-LFS Assay for Detection of Capsulated and Non-CapsulatedFrontiers in cellular and infection microbiology · 2022Article
- Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae.Frontiers in cellular and infection microbiology · 2022Article
- Parental preference for Haemophilus influenzae type b vaccination in Zhejiang Province, China: A discrete choice experiment.Frontiers in public health · 2022Article
- Particulate air pollution and respiratory Haemophilus influenzae infection in Mianyang, southwest China.Environmental science and pollution research international · 2021Article
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8 authors at 2 institutions in 1 country.
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Abstract
backgroundHaemophilus influenzae (H. influenzae) is one of the most important pathogenic bacteria causing respiratory tract infection diseases in children. There are two main types of H. influenzae, encapsulated H. influenzae and nontypeable H. influenzae (NTHi). Serotype b of H. influenzae (Hib) used to be the main epidemic type of H. influenzae, causing the invasive infection. However, the epidemiology of invasive H. influenzae disease has changed substantially, and most invasive diseases are now caused by NTHi and other serotypes of H. influenzae. The aim of this study was to determine the main epidemic strains of H. influenzae in Zhejiang Province in China, and establish a one-step multiplex PCR assay to distinguish H. influenzae from other bacteria associated with respiratory tract infections, and distinguish encapsulated H. influenzae from NTHi.
methodIn this study, bacterial culture and serum agglutination testing were used to determine the most prevalent serotype of H. influenzae, and the results have served as a gold standard for clinical diagnosis. We also designed a one-step multiplex PCR assay using several kinds of standard strains of respiratory tract infection bacteria, to examine the stability, specificity, and detection limit of the PCR assays. We then used 1514 nasopharyngeal secretion (NPS) samples collected from children with respiratory tract infections to verify the specificity and sensitivity of the PCR assay.
resultsThe bacterial culture and serum agglutination test results showed that the positive rates of H. influenzae and encapsulated H. influenzae were 18.49 and 1.18%, respectively. The PCR results showed that the detection limit of the multiplex PCR assay was 1.89 × 10
conclusionsAccording to our study, the most prevalent H. influenzae subtype in Zhejiang Province was NTHi, account for 93.57%; the one-step multiplex PCR assay we established can be used as the differential detection of clinical H. influenzae strains, replacing routine bacterial culture and serum agglutination testing.
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