ArticlePLoS neglected tropical diseases2018
Estimating the effects of variation in viremia on mosquito susceptibility, infectiousness, and R0 of Zika in Aedes aegypti.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Evaluation of mosquito transmission of equine hepacivirus in laboratory and field studies.Journal of medical entomology · 2026Article
- Detritus and larval competition influence phenotypic traits, nutrient stoichiometry, and vector competence for dengue virus in Aedes aegypti.Parasites & vectors · 2026Article
- Entomological Surveillance and Vector Competence: A Crucial Front in Dengue Prevention.Current topics in microbiology and immunology · 2026Review
- Estimating per-infection cost and burden for dengue and Zika as a function of antibody-dependent enhancement.PLoS neglected tropical diseases · 2025Article
- NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts.Nature communications · 2025Article
- Investigating the dose-dependency of the midgut escape barrier using a mechanistic model of within-mosquito dengue virus population dynamics.PLoS pathogens · 2024Article
- Mechanistic modelling of within-mosquito viral dynamics: Insights into infection and dissemination patterns.PLoS computational biology · 2023Article
- The significance of mosquito saliva in arbovirus transmission and pathogenesis in the vertebrate host.One health (Amsterdam, Netherlands) · 2023Article
- Factors Affecting Arbovirus Midgut Escape in Mosquitoes.Pathogens (Basel, Switzerland) · 2023Review
- Not all mosquitoes are created equal: A synthesis of vector competence experiments reinforces virus associations of Australian mosquitoes.PLoS neglected tropical diseases · 2022Article
- Heterogeneity of Rift Valley fever virus transmission potential across livestock hosts, quantified through a model-based analysis of host viral load and vector infection.PLoS computational biology · 2022Article
- Quantitative age grading of mosquitoes using surface-enhanced Raman spectroscopy.Analytical science advances · 2022Article
- Starvation at the larval stage increases the vector competence of Aedes aegypti females for Zika virus.PLoS neglected tropical diseases · 2021Article
- Persistence of Zika virus RNA in the epididymis of the murine male reproductive tract.Virology · 2021Article
- Follow-Up Household Serosurvey in Northeast Brazil for Zika Virus: Sexual Contacts of Index Patients Have the Highest Risk for Seropositivity.The Journal of infectious diseases · 2021Article
- Recent African strains of Zika virus display higher transmissibility and fetal pathogenicity than Asian strains.Nature communications · 2021Article
- Modeling intra-mosquito dynamics of Zika virus and its dose-dependence confirms the low epidemic potential of Aedes albopictus.PLoS pathogens · 2020Article
- LowViruses · 2020Article
- Age influences the thermal suitability ofProceedings. Biological sciences · 2020Article
- Insecticide resistance, fitness and susceptibility to Zika infection of an interbred Aedes aegypti population from Rio de Janeiro, Brazil.Parasites & vectors · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zika virus (ZIKV) is an arbovirus primarily transmitted by Aedes mosquitoes. Like most viral infections, ZIKV viremia varies over several orders of magnitude, with unknown consequences for transmission. To determine the effect of viral concentration on ZIKV transmission risk, we exposed field-derived Ae. aegypti mosquitoes to four doses (10(3), 10(4), 10(5), 10(6) PFU/mL) representative of potential variation in the field. We demonstrate that increasing ZIKV dose in the blood-meal significantly increases the probability of mosquitoes becoming infected, and consequently disseminating virus and becoming infectious. Additionally, we observed significant interactions between dose and days post-infection on dissemination and overall transmission efficiency, suggesting that variation in ZIKV dose affects the rates of midgut escape and salivary gland invasion. We did not find significant effects of dose on mosquito mortality. We also demonstrate that detecting virus using RT-qPCR approaches rather than plaque assays potentially over-estimates key transmission parameters, including the time at which mosquitoes become infectious and viral burden. Finally, using these data to parameterize an R0 model, we showed that increasing viremia from 10(4) to 10(6) PFU/mL increased relative R0 3.8-fold, demonstrating that variation in viremia substantially affects transmission risk.
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