ArticlePlant biotechnology journal2019
Gene regulatory networks for lignin biosynthesis in switchgrass (Panicum virgatum).
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 100 citations in OpenAlex.
- Genome-wide analysis of the R2R3-MYB family reveals potential regulators of lignin and tricin metabolism in the model grass Setaria viridis.Molecular genetics and genomics : MGG · 2026Article
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- Multifaceted roles and regulatory mechanisms of MYB transcription factors in plant development, secondary metabolism, and stress adaptation: current insights and future prospects.GM crops & food · 2025Review
- Advances in lignocellulosic feedstocks for bioenergy and bioproducts.Nature communications · 2025Review
- Mechanistic insights into nutrient profiles, cellulose, and hemicellulose dynamics in red and greenFrontiers in plant science · 2025Article
- Transcriptome-Based Screening of Candidate Low-Temperature-Associated Genes and Analysis of theInternational journal of molecular sciences · 2024Article
- StCoExpNet: a global co-expression network analysis facilitates identifying genes underlying agronomic traits in potatoes.Plant cell reports · 2024Article
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- Small secreted proteins and exocytosis regulators: do they go along?Plant signaling & behavior · 2023Article
- Integrated microRNA and whole-transcriptome sequencing reveals the involvement of small and long non-coding RNAs in the fiber growth of ramie plant.BMC genomics · 2023Article
- Comparative Transcriptome Profiling Reveals Two WRKY Transcription Factors Positively Regulating Polysaccharide Biosynthesis inInternational journal of molecular sciences · 2023Article
- The Overexpression ofInternational journal of molecular sciences · 2023Article
- Transgenic ZmMYB167 Miscanthus sinensis with increased lignin to boost bioenergy generation for the bioeconomy.Biotechnology for biofuels and bioproducts · 2023Article
- Integrative analysis of metabolome, proteome, and transcriptome for identifying genes influencing total lignin content inFrontiers in plant science · 2023Article
- Characterization, Expression Profiling, and Biochemical Analyses of theInternational journal of molecular sciences · 2022Article
- PlantNexus: A Gene Co-expression Network Database and Visualization Tool for Barley and Sorghum.Plant & cell physiology · 2022Article
- Comparative Transcriptome and Metabolome Analysis of Resistant and SusceptibleFrontiers in plant science · 2022Article
- Distinct and Overlapping Functions ofInternational journal of molecular sciences · 2021Article
- Towards a Cardoon (International journal of molecular sciences · 2021Article
- Precursor biosynthesis regulation of lignin, suberin and cutin.Protoplasma · 2021Review
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 2 countries.
Funding
Abstract
Cell wall recalcitrance is the major challenge to improving saccharification efficiency in converting lignocellulose into biofuels. However, information regarding the transcriptional regulation of secondary cell wall biogenesis remains poor in switchgrass (Panicum virgatum), which has been selected as a biofuel crop in the United States. In this study, we present a combination of computational and experimental approaches to develop gene regulatory networks for lignin formation in switchgrass. To screen transcription factors (TFs) involved in lignin biosynthesis, we developed a modified method to perform co-expression network analysis using 14 lignin biosynthesis genes as bait (target) genes. The switchgrass lignin co-expression network was further extended by adding 14 TFs identified in this study, and seven TFs identified in previous studies, as bait genes. Six TFs (PvMYB58/63, PvMYB42/85, PvMYB4, PvWRKY12, PvSND2 and PvSWN2) were targeted to generate overexpressing and/or down-regulated transgenic switchgrass lines. The alteration of lignin content, cell wall composition and/or plant growth in the transgenic plants supported the role of the TFs in controlling secondary wall formation. RNA-seq analysis of four of the transgenic switchgrass lines revealed downstream target genes of the secondary wall-related TFs and crosstalk with other biological pathways. In vitro transactivation assays further confirmed the regulation of specific lignin pathway genes by four of the TFs. Our meta-analysis provides a hierarchical network of TFs and their potential target genes for future manipulation of secondary cell wall formation for lignin modification in switchgrass.
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