ArticlePlant biotechnology journal2019
RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family.
Article in Plant biotechnology journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- A neprilysin effector from the pinewood nematode Bursaphelenchus xylophilus targets pine caffeic acid O-methyltransferase and inhibits lignin biosynthesis.Pest management science · 2026Article
- Boosting photosynthesis opens new opportunities for agriculture sustainability and circular economy: The BEST-CROP research and innovation action.The Plant journal : for cell and molecular biology · 2025Review
- Rhizophagus Irregularis regulates flavonoids metabolism in paper mulberry roots under cadmium stress.Mycorrhiza · 2024Article
- Caffeic AcidInternational journal of molecular sciences · 2024Article
- Melatonin Role in Plant Growth and Physiology under Abiotic Stress.International journal of molecular sciences · 2023Review
- Development of an in vitro regeneration system from immature inflorescences and CRISPR/Cas9-mediated gene editing in sudangrass.Journal, genetic engineering & biotechnology · 2023Article
- Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants.Plant physiology · 2023Article
- Natural variation inFrontiers in plant science · 2023Article
- Cas9/gRNA-Mediated Mutations inInternational journal of molecular sciences · 2022Article
- RNA-Seq and Iso-Seq Reveal the Important Role ofGenes · 2022Article
- High temperature increased lignin contents of poplar (Frontiers in genetics · 2022Article
- Downregulation of barley ferulate 5-hydroxylase dramatically alters straw lignin structure without impact on mechanical properties.Frontiers in plant science · 2022Article
- Genome-Wide Identification and Characterization of GhCOMT Gene Family during Fiber Development and Verticillium Wilt Resistance in Cotton.Plants (Basel, Switzerland) · 2021Article
- The role of watermelon caffeic acid O-methyltransferase (ClCOMT1) in melatonin biosynthesis and abiotic stress tolerance.Horticulture research · 2021Article
- Phenylpropanoid Biosynthesis Gene Expression Precedes Lignin Accumulation During Shoot Development in Lowland and Upland Switchgrass Genotypes.Frontiers in plant science · 2021Article
- Tricin Biosynthesis and Bioengineering.Frontiers in plant science · 2021Review
- Association mapping identifies quantitative trait loci (QTL) for digestibility in rice straw.Biotechnology for biofuels · 2020Article
- OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls.Scientific reports · 2019Article
- Overexpression ofInternational journal of molecular sciences · 2019Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 5 countries.
Funding
Abstract
Caffeic acid O-methyltransferase (COMT), the lignin biosynthesis gene modified in many brown-midrib high-digestibility mutants of maize and sorghum, was targeted for downregulation in the small grain temperate cereal, barley (Hordeum vulgare), to improve straw properties. Phylogenetic and expression analyses identified the barley COMT orthologue(s) expressed in stems, defining a larger gene family than in brachypodium or rice with three COMT genes expressed in lignifying tissues. RNAi significantly reduced stem COMT protein and enzyme activity, and modestly reduced stem lignin content while dramatically changing lignin structure. Lignin syringyl-to-guaiacyl ratio was reduced by ~50%, the 5-hydroxyguaiacyl (5-OH-G) unit incorporated into lignin at 10--15-fold higher levels than normal, and the amount of p-coumaric acid ester-linked to cell walls was reduced by ~50%. No brown-midrib phenotype was observed in any RNAi line despite significant COMT suppression and altered lignin. The novel COMT gene family structure in barley highlights the dynamic nature of grass genomes. Redundancy in barley COMTs may explain the absence of brown-midrib mutants in barley and wheat. The barley COMT RNAi lines nevertheless have the potential to be exploited for bioenergy applications and as animal feed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.