Evidence map›Paper›PMID 30131909›Full record

ReviewApplications in plant sciences2018

Generating DNA sequence data with limited resources for molecular biology: Lessons from a barcoding project in Indonesia.

Gillian H Dean, Rani Asmarayani, Marlina Ardiyani, Yessi Santika, Teguh Triono, Sarah Mathews, Campbell O Webb

Abstract readReview
In one paragraph

Review in Applications in plant sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Gillian H DeanDepartment of Botany University of British Columbia Vancouver V6T1Z4 British Columbia Canada.ORCID 0000-0003-1126-8355
Rani AsmarayaniHerbarium Bogoriense Botany Division Research Center for Biology Indonesian Institute of Sciences (LIPI) Cibinong 16911 Bogor West Java Indonesia.
Marlina ArdiyaniHerbarium Bogoriense Botany Division Research Center for Biology Indonesian Institute of Sciences (LIPI) Cibinong 16911 Bogor West Java Indonesia.
Yessi SantikaHerbarium Bogoriense Botany Division Research Center for Biology Indonesian Institute of Sciences (LIPI) Cibinong 16911 Bogor West Java Indonesia.
Teguh TrionoHerbarium Bogoriense Botany Division Research Center for Biology Indonesian Institute of Sciences (LIPI) Cibinong 16911 Bogor West Java Indonesia.
Sarah MathewsArnold Arboretum of Harvard University Boston Massachusetts 02131 USA.ORCID 0000-0002-5518-7541
Campbell O WebbArnold Arboretum of Harvard University Boston Massachusetts 02131 USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The advent of the DNA sequencing age has led to a revolution in biology. The rapid and cost-effective generation of high-quality sequence data has transformed many fields, including those focused on discovering species and surveying biodiversity, monitoring movement of biological materials, forensic biology, and disease diagnostics. There is a need to build capacity to generate useful sequence data in countries with limited historical access to laboratory resources, so that researchers can benefit from the advantages offered by these data. Commonly used molecular techniques such as DNA extraction, PCR, and DNA sequencing are within the reach of small laboratories in many countries, with the main obstacles to successful implementation being lack of funding and limited practical experience. Here we describe a successful approach that we developed to obtain DNA sequence data during a small DNA barcoding project in Indonesia.

Indexed as

DNA barcodingDNA sequence datalimited resourcesmolecular biology

Identifiers

PMID30131909
PMCPMC6055555

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.