Evidence map›Paper›PMID 30131890›Full record

ArticleApplications in plant sciences2018

Target sequence capture of nuclear-encoded genes for phylogenetic analysis in ferns.

Paul G Wolf, Tanner A Robison, Matthew G Johnson, Michael A Sundue, Weston L Testo, Carl J Rothfels

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Article in Applications in plant sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Paul G WolfEcology Center and Department of Biology Utah State University Logan Utah 84322 USA.ORCID 0000-0002-4317-6976
Tanner A RobisonEcology Center and Department of Biology Utah State University Logan Utah 84322 USA.ORCID 0000-0002-1629-9786
Matthew G JohnsonDepartment of Biological Sciences Texas Tech University Lubbock Texas 79409 USA.ORCID 0000-0002-1958-6334
Michael A SunduePringle Herbarium Department of Plant Biology University of Vermont Burlington Vermont 05405 USA.ORCID 0000-0003-1568-150X
Weston L TestoPringle Herbarium Department of Plant Biology University of Vermont Burlington Vermont 05405 USA.ORCID 0000-0003-3194-5763
Carl J RothfelsUniversity Herbarium and Department of Integrative Biology University of California Berkeley California 94720 USA.ORCID 0000-0002-6605-1770

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premise of the studyUntil recently, most phylogenetic studies of ferns were based on chloroplast genes. Evolutionary inferences based on these data can be incomplete because the characters are from a single linkage group and are uniparentally inherited. These limitations are particularly acute in studies of hybridization, which is prevalent in ferns; fern hybrids are common and ferns are able to hybridize across highly diverged lineages, up to 60 million years since divergence in one documented case. However, it not yet clear what effect such hybridization has on fern evolution, in part due to a paucity of available biparentally inherited (nuclear-encoded) markers.

methodsWe designed oligonucleotide baits to capture 25 targeted, low-copy nuclear markers from a sample of 24 species spanning extant fern diversity.

resultsMost loci were successfully sequenced from most accessions. Although the baits were designed from exon (transcript) data, we successfully captured intron sequences that should be useful for more focused phylogenetic studies. We present phylogenetic analyses of the new target sequence capture data and integrate these into a previous transcript-based data set. DISCUSSION: We make our bait sequences available to the community as a resource for further studies of fern phylogeny.

Indexed as

fernsHybPiperhybridizationHyb‐Seqphylogenytarget sequence capture

Identifiers

PMID30131890
PMCPMC5991577

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.