ArticleApplications in plant sciences2018
HPLC-MS detection of pyrrolizidine alkaloids and their
Article in Applications in plant sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 21 citations in OpenAlex.
- Sequestration and the Extended Museum Specimen: Effects of Time and Preparation Methodology.Journal of chemical ecology · 2025Article
- Pyrrolizidine alkaloids cause cell cycle and DNA damage repair defects as analyzed by transcriptomics in cytochrome P450 3A4-overexpressing HepG2 clone 9 cells.Cell biology and toxicology · 2022Article
- HPLC-MS detection of pyrrolizidine alkaloids and theirApplications in plant sciences · 2018Article
- Hundred Fifty Years of Herbarium Collections Provide a Reliable Resource of Volatile Terpenoid Profiles Showing Strong Species Effect in Four Medicinal Species ofFrontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
premise of the studyUnderstanding the phylogenetic distribution of defensive plant secondary metabolites is essential to the macroevolutionary study of chemically mediated plant-animal interactions. The chemical ecology of pyrrolizidine alkaloids (PAs) has been extensively studied in a number of plant-herbivore systems, including Apocynaceae (the milkweed and dogbane family) and Danainae (the milkweed and clearwing butterflies). A systematic survey is necessary to establish a detailed understanding of their occurrence across Apocynaceae. A survey of this species-rich, mainly tropical and subtropical family will rely heavily on small tissue samples removed from herbarium specimens, some of which will be very old and/or preserved with alcohols or mercuric chloride.
methodsWe optimized PA extraction methods from small leaf fragments of recently collected silica-dried leaves of the PA-positive
resultsFifteen seconds of leaf crushing followed by 24 h of extraction were optimal for PA free-base and
conclusionsPAs can be reliably detected in small tissue samples from herbarium specimens up to 167 years old, including specimens that had been treated with alcohol or mercury salts. The variability of PA presence among herbarium specimens of
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