ArticleScientific reports2018
mKast is dispensable for normal development and sexual maturation of the male European honeybee.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 33 citations in OpenAlex.
- Targeted Gene Editing in Honey Bees Using Liposome-Based CRISPR-Cas9.Biochemical genetics · 2026Article
- Butterfly brains change in morphology and in gene splicing patterns after brief pheromone exposure.BMC biology · 2026Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
- Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus.Development (Cambridge, England) · 2025Article
- Unique spatially and temporary-regulated/sex-specific expression of a long ncRNA, Nb-1, suggesting its pleiotropic functions associated with honey bee lifecycle.Scientific reports · 2024Article
- Honey bee functional genomics using symbiont-mediated RNAi.Nature protocols · 2023Review
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- Unmanned Aerial Vehicle for Laser Based Biomedical Sensor Development and Examination of Device Trajectory.Sensors (Basel, Switzerland) · 2022Article
- Review
- Genetic basis of chemical communication in eusocial insects.Genes & development · 2021Review
- A simple method for ex vivo honey bee cell culture capable of in vitro gene expression analysis.PloS one · 2021Article
- Review
- Review
- High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee (G3 (Bethesda, Md.) · 2019Article
- Feminizer and doublesex knock-outs cause honey bees to switch sexes.PLoS biology · 2019Article
- A genetic switch for worker nutrition-mediated traits in honeybees.PLoS biology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The European honeybee (Apis mellifera L.) exhibits various social behaviors. The molecular and neural mechanisms underlying these behaviors have long been explored, but causal relations between genes or neurons and behaviors remain to be elucidated because effective gene manipulation methods in the honeybee have not been available until recently. We recently established a basic technology to produce mutant honeybee drones using CRISPR/Cas9. Here we produced mutant drones using CRISPR/Cas9 targeting mKast, which is preferentially expressed in a certain subtype of class I Kenyon cells that comprise the mushroom bodies in the honeybee brain. By immunoblot analysis, we showed that mKast protein expression was completely lost in the mutant drone heads. In addition, during the production process of homozygous mutant workers, we demonstrated that heterozygous mutant workers could be produced by artificial insemination of wild-type queens with the sperm of mutant drones, indicating that mKast mutant drones were sexually mature. These results demonstrate that mKast is dispensable for normal development and sexual maturation in drone honeybees, and allow us to proceed with the production of homozygous mutant workers for the analysis of a particular gene by gene knockout in the future.
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