ReviewProtein and peptide letters2018
An Overview of Current Methods to Confirm Protein-Protein Interactions.
Review in Protein and peptide letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Yeast-two-hybrid based high-throughput screening to discover SARS-CoV-2 fusion inhibitors by targeting the HR1/HR2 interaction.Acta pharmaceutica Sinica. B · 2025Article
- Bacterial two-hybrid systems evolved: innovations for protein-protein interaction research.Journal of bacteriology · 2025Review
- Spatially resolved subcellular protein-protein interactomics in drug-perturbed lung-cancer cultures and tissues.Nature biomedical engineering · 2025Article
- Creating Chemiluminescence Signature Arrays Coupled with Machine Learning for Alzheimer's Disease Serum Diagnosis.Research (Washington, D.C.) · 2025Article
- Identifying Protein-Protein Interaction or Protein Phosphorylation by Proximity Ligation Assay (PLA) in Endothelial Cells.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ligand Gaussian Accelerated Molecular Dynamics 3 (LiGaMD3): Improved Calculations of Binding Thermodynamics and Kinetics of Both Small Molecules and Flexible Peptides.Journal of chemical theory and computation · 2024Article
- Ligand Gaussian accelerated Molecular Dynamics 3 (LiGaMD3): Improved Calculations of Binding Thermodynamics and Kinetics of Both Small Molecules and Flexible Peptides.bioRxiv : the preprint server for biology · 2024Article
- High-Throughput Interactome Determination via Sulfur Anomalous Scattering.The journal of physical chemistry letters · 2024Article
- Searching for EGF Fragments Recreating the Outer Sphere of the Growth Factor Involved in Receptor Interactions.International journal of molecular sciences · 2024Article
- Prediction of Human Papillomavirus-Host Oncoprotein Interactions Using Deep Learning.Bioinformatics and biology insights · 2024Article
- Accelerating Molecular Dynamics Simulations for Drug Discovery.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Phase separation-based visualization of protein-protein interactions and kinase activities in plants.The Plant cell · 2023Article
- A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis.Methods and protocols · 2023Article
- Ligand Gaussian Accelerated Molecular Dynamics 2 (LiGaMD2): Improved Calculations of Ligand Binding Thermodynamics and Kinetics with Closed Protein Pocket.Journal of chemical theory and computation · 2023Article
- Methods to Assess Chemokine Binding and Anti-chemotactic Activity of Virus Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics.Methods in molecular biology (Clifton, N.J.) · 2023Article
- GST-IVTT pull-down: a fast and versatile in vitro method for validating and mapping protein-protein interactions.FEBS open bio · 2022Article
- Scaling-up a fragment-based protein-protein interaction method using a human reference interaction set.Proteins · 2022Article
- Protein-Protein Interaction-Gaussian Accelerated Molecular Dynamics (PPI-GaMD): Characterization of Protein Binding Thermodynamics and Kinetics.Journal of chemical theory and computation · 2022Article
- YdfD, a Lysis Protein of the Qin Prophage, Is a Specific Inhibitor of the IspG-Catalyzed Step in the MEP Pathway ofInternational journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe research field of protein-protein interactions is interdisciplinary and specialized field that spans all aspects of biology, physics and chemistry. Therefore, in order to discuss the protein-protein interaction in detail and rigorously, it is desirable to integrate knowledge and methods of many related fields including boundary areas such as biochemistry, biophysics and physical chemistry in addition to biology, physics and chemistry.
objectiveThe purpose of this review is to overview current methods to confirm protein-protein interactions. Furthermore, I discuss future prospects of methodology based on current status.
resultsIt is often necessary to integrate, combine and validate multiple results from various methods to understand protein-protein interactions in detail.
conclusionIt might be desirable for the addition of tags, labeling, and immobilization to solid phases to be unnecessary, and to obtain information on affinity, kinetics, and structure via the analytical method for protein-protein interactions. Therefore, I argue that novel methods based on principles that have already been sufficiently studied in physics or chemistry, but insufficiently applied to the life sciences, should be established to further develop the study of protein-protein interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.