Evidence map›Paper›PMID 30129022›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Using FM Dyes to Monitor Clathrin-Mediated Endocytosis in Primary Neuronal Culture.

Michael A Cousin, Sarah L Gordon, Karen J Smillie

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. TUSC3 regulates ERMA-mediated MgNature communications · 2025
    Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Michael A CousinCentre for Discovery Brain Sciences, Edinburgh Medical School: Biomedical Sciences, University of Edinburgh, Edinburgh, UK.
Sarah L GordonFlorey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC, Australia.
Karen J SmillieCentre for Discovery Brain Sciences, Edinburgh Medical School: Biomedical Sciences, University of Edinburgh, Edinburgh, UK. K.Smillie@ed.ac.uk.

Funding

Medical Research Council G1002117
6 · The paper itself

Abstract

This protocol utilizes lipophilic FM dyes to monitor membrane recycling in real time. FM dyes are virtually nonfluorescent in solution but when membrane bound are intensely fluorescent, combined with the flexibility of different emission wavelengths make these dyes an excellent choice for investigating clathrin-mediated endocytosis, among other membrane trafficking and recycling pathways.

Indexed as

EndocytosisAnimalsCells, CulturedClathrinFluorescent DyesMicroscopy, FluorescenceNeuronsClathrinFluorescent DyesClathrin-mediated endocytosisEpifluorescenceFM dyesLive cell imagingPrimary neuronal culture

Identifiers

PMID30129022

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.