Evidence map›Paper›PMID 30129014›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-Molecule Analysis.

Till Böcking, Srigokul Upadhyayula, Iris Rapoport, Benjamin R Capraro, Tom Kirchhausen

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Till BöckingARC Centre of Excellence in Advanced Molecular Imaging and EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Australia. till.boecking@unsw.edu.au.
Srigokul UpadhyayulaDepartment of Cell Biology, Harvard Medical School; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA. gokul@tklab.hms.harvard.edu.
Iris RapoportDepartment of Cell Biology, Harvard Medical School; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
Benjamin R CapraroDepartment of Cell Biology, Harvard Medical School; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
Tom KirchhausenDepartment of Cell Biology, Harvard Medical School; Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA. kirchhau@crystal.harvard.edu.
Boston Children's Hospital · USHarvard University · USUNSW Sydney · AU

Funding

Dynamics of Clathrin Coat Formation in CellsR01GM075252 · NIGMS · IMMUNE DISEASE INSTITUTE, INC. · PI KIRCHHAUSEN, TOMAS · 2005 to 2018
$4.6M
NIGMS NIH HHS R01 GM075252
6 · The paper itself

Abstract

The disassembly of the clathrin lattice surrounding coated vesicles is the obligatory last step in their life cycle. It is mediated by the coordinated recruitment of auxilin and Hsc70, an ATP-driven molecular clamp. Here, we describe the preparation of reagents and the single-particle fluorescence microscopy imaging assay in which we visualize directly the Hsc70-driven uncoating of synthetic clathrin coats or clathrin-coated vesicles.

Indexed as

Microscopy, FluorescenceMolecular ImagingAuxilinsBiological TransportChromatography, AffinityClathrinClathrin-Coated VesiclesHSC70 Heat-Shock ProteinsLiposomesMicrofluidic Analytical TechniquesRecombinant ProteinsStaining and LabelingAuxilinsClathrinHSC70 Heat-Shock ProteinsLiposomesRecombinant ProteinsAuxilinClathrin-coated vesicleClathrin uncoatingMicrofluidicsTIRF microscopy

Identifiers

PMID30129014
PMCPMC6669891
OpenAlexW2888292556

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.