Evidence map›Paper›PMID 30129011›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Imaging "Hot-Wired" Clathrin-Mediated Endocytosis.

Laura A Wood, Stephen J Royle

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Laura A WoodCentre for Mechanochemical Cell Biology, Warwick Medical School, Coventry, UK.
Stephen J RoyleCentre for Mechanochemical Cell Biology, Warwick Medical School, Coventry, UK. s.j.royle@warwick.ac.uk.
University of Bath · GBUniversity of Warwick · GB

Funding

Medical Research Council MR/J003964/1
6 · The paper itself

Abstract

Clathrin-mediated endocytosis (CME) occurs continuously at the plasma membrane of eukaryotic cells. However, when a vesicle forms and what cargo it contains are unpredictable. We recently developed a system to trigger CME on-demand. This means that we can control when endocytosis is triggered and the design means that the cargo that is internalized is predetermined. The method is called hot-wired CME because several steps and proteins are bypassed in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.

Indexed as

Molecular ImagingClathrinClathrin-Coated VesiclesEndocytosisFluorescent Antibody TechniqueHeLa CellsHumansImage Processing, Computer-AssistedImmunohistochemistryMicroscopy, ConfocalClathrinChemically induced dimerizationClathrinEndocytosisImage analysisImmunofluorescenceLive cell imaging

Identifiers

PMID30129011
OpenAlexW2888057770

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.